1lgy
From Proteopedia
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| - | [[Image:1lgy.jpg|left|200px]] | + | [[Image:1lgy.jpg|left|200px]] |
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| - | '''LIPASE II FROM RHIZOPUS NIVEUS''' | + | {{Structure |
| + | |PDB= 1lgy |SIZE=350|CAPTION= <scene name='initialview01'>1lgy</scene>, resolution 2.2Å | ||
| + | |SITE= <scene name='pdbsite=CAT:Catalytic+Site'>CAT</scene> | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''LIPASE II FROM RHIZOPUS NIVEUS''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1LGY is a [ | + | 1LGY is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rhizopus_niveus Rhizopus niveus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LGY OCA]. |
==Reference== | ==Reference== | ||
| - | The crystal structure of lipase II from Rhizopus niveus at 2.2 A resolution., Kohno M, Funatsu J, Mikami B, Kugimiya W, Matsuo T, Morita Y, J Biochem. 1996 Sep;120(3):505-10. PMID:[http:// | + | The crystal structure of lipase II from Rhizopus niveus at 2.2 A resolution., Kohno M, Funatsu J, Mikami B, Kugimiya W, Matsuo T, Morita Y, J Biochem. 1996 Sep;120(3):505-10. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8902613 8902613] |
[[Category: Rhizopus niveus]] | [[Category: Rhizopus niveus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: lipase]] | [[Category: lipase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:30:46 2008'' |
Revision as of 10:30, 20 March 2008
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| , resolution 2.2Å | |||||||
|---|---|---|---|---|---|---|---|
| Sites: | |||||||
| Activity: | Triacylglycerol lipase, with EC number 3.1.1.3 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
LIPASE II FROM RHIZOPUS NIVEUS
Overview
The crystal and molecular structure of Lipase II from Rhizopus niveus was analyzed using X-ray single crystal diffraction data at a resolution of 2.2 A. The structure was refined to an R-factor of 0.19 for all available data. This lipase was purified and crystallized as Lipase I, which contains two polypeptide chains combined through non-covalent interaction. However, during crystal growth, Lipase I was converted to Lipase II, which consists of a single polypeptide chain of 269 amino acid residues, by limited proteolysis. The structure of Lipase II shows a typical alpha/beta hydrolase fold containing the so-called nucleophilic elbow. The catalytic center of this enzyme is analogous to those of other neutral lipases and serine proteases. This catalytic center is sheltered by an alpha-helix lid, which appears in neutral lipases, opening the active site at the oil-water interface.
About this Structure
1LGY is a Single protein structure of sequence from Rhizopus niveus. Full crystallographic information is available from OCA.
Reference
The crystal structure of lipase II from Rhizopus niveus at 2.2 A resolution., Kohno M, Funatsu J, Mikami B, Kugimiya W, Matsuo T, Morita Y, J Biochem. 1996 Sep;120(3):505-10. PMID:8902613
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