1lhr

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[[Image:1lhr.jpg|left|200px]]<br /><applet load="1lhr" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1lhr.jpg|left|200px]]
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caption="1lhr, resolution 2.6&Aring;" />
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'''Crystal Structure of Pyridoxal Kinase complexed with ATP'''<br />
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{{Structure
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|PDB= 1lhr |SIZE=350|CAPTION= <scene name='initialview01'>1lhr</scene>, resolution 2.6&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene> and <scene name='pdbligand=ATP:ADENOSINE-5'-TRIPHOSPHATE'>ATP</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Pyridoxal_kinase Pyridoxal kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.35 2.7.1.35]
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|GENE=
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}}
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'''Crystal Structure of Pyridoxal Kinase complexed with ATP'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1LHR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Ovis_aries Ovis aries] with <scene name='pdbligand=ZN:'>ZN</scene>, <scene name='pdbligand=K:'>K</scene> and <scene name='pdbligand=ATP:'>ATP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Pyridoxal_kinase Pyridoxal kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.35 2.7.1.35] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LHR OCA].
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1LHR is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Ovis_aries Ovis aries]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LHR OCA].
==Reference==
==Reference==
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Crystal structure of brain pyridoxal kinase, a novel member of the ribokinase superfamily., Li MH, Kwok F, Chang WR, Lau CK, Zhang JP, Lo SC, Jiang T, Liang DC, J Biol Chem. 2002 Nov 29;277(48):46385-90. Epub 2002 Sep 15. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12235162 12235162]
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Crystal structure of brain pyridoxal kinase, a novel member of the ribokinase superfamily., Li MH, Kwok F, Chang WR, Lau CK, Zhang JP, Lo SC, Jiang T, Liang DC, J Biol Chem. 2002 Nov 29;277(48):46385-90. Epub 2002 Sep 15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12235162 12235162]
[[Category: Ovis aries]]
[[Category: Ovis aries]]
[[Category: Pyridoxal kinase]]
[[Category: Pyridoxal kinase]]
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[[Category: complexed with atp]]
[[Category: complexed with atp]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:45:04 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:31:02 2008''

Revision as of 10:31, 20 March 2008

Image:1lhr.jpg


PDB ID 1lhr

Drag the structure with the mouse to rotate
, resolution 2.6Å
Ligands: , and
Activity: Pyridoxal kinase, with EC number 2.7.1.35
Coordinates: save as pdb, mmCIF, xml



Crystal Structure of Pyridoxal Kinase complexed with ATP


Overview

The three-dimensional structures of brain pyridoxal kinase and its complex with the nucleotide ATP have been elucidated in the dimeric form at 2.1 and 2.6 A, respectively. Results have shown that pyridoxal kinase, as an enzyme obeying random sequential kinetics in catalysis, does not possess a lid shape structure common to all kinases in the ribokinase superfamily. This finding has been shown to be in line with the condition that pyridoxal kinase binds substrates with variable sizes of chemical groups at position 4 of vitamin B(6) and its derivatives. In addition, the enzyme contains a 12-residue peptide loop in the active site for the prevention of premature hydrolysis of ATP. Conserved amino acid residues Asp(118) and Tyr(127) in the peptide loop could be moved to a position covering the nucleotide after its binding so that its chance to hydrolyze in the aqueous environment of the active site was reduced. With respect to the evolutionary trend of kinase enzymes, the existence of this loop in pyridoxal kinase could be classified as an independent category in the ribokinase superfamily according to the structural feature found and mechanism followed in catalysis.

About this Structure

1LHR is a Single protein structure of sequence from Ovis aries. Full crystallographic information is available from OCA.

Reference

Crystal structure of brain pyridoxal kinase, a novel member of the ribokinase superfamily., Li MH, Kwok F, Chang WR, Lau CK, Zhang JP, Lo SC, Jiang T, Liang DC, J Biol Chem. 2002 Nov 29;277(48):46385-90. Epub 2002 Sep 15. PMID:12235162

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