3e1h

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[[Image:3e1h.png|left|200px]]
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==Crystal structure of a type III polyketide synthase PKSIIINc from Neurospora crassa==
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<StructureSection load='3e1h' size='340' side='right' caption='[[3e1h]], [[Resolution|resolution]] 2.58&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3e1h]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Neurospora_crassa Neurospora crassa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3E1H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3E1H FirstGlance]. <br>
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</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Naringenin-chalcone_synthase Naringenin-chalcone synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.74 2.3.1.74] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3e1h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3e1h OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3e1h RCSB], [http://www.ebi.ac.uk/pdbsum/3e1h PDBsum]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/e1/3e1h_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Microbial type III polyketide synthases (PKSs) have revealed remarkable mechanistic as well as functional versatility. Recently, a type III PKS homolog from Azotobacter has been implicated in the biosynthesis of resorcinolic lipids, thus adding a new functional significance to this class of proteins. Here, we report the structural and mutational investigations of a novel type III PKS protein from Neurospora crassa involved in the biosynthesis of resorcinolic metabolites by utilizing long chain fatty acyl-CoAs. The structure revealed a long hydrophobic tunnel responsible for its fatty acyl chain length specificity resembling that of PKS18, a mycobacterial type III PKS. Structure-based mutational studies to block the tunnel not only altered the fatty acyl chain specificity but also resulted in change of cyclization pattern affecting the product profile. This first structural characterization of a resorcinolic lipid synthase provides insights into the coordinated functioning of cyclization and a substrate-binding pocket, which shows mechanistic intricacy underlying type III PKS catalysis.
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{{STRUCTURE_3e1h| PDB=3e1h | SCENE= }}
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Structural insights into biosynthesis of resorcinolic lipids by a type III polyketide synthase in Neurospora crassa.,Goyal A, Saxena P, Rahman A, Singh PK, Kasbekar DP, Gokhale RS, Sankaranarayanan R J Struct Biol. 2008 Jun;162(3):411-21. Epub 2008 Mar 30. PMID:18462950<ref>PMID:18462950</ref>
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===Crystal structure of a type III polyketide synthase PKSIIINc from Neurospora crassa===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_18462950}}
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== References ==
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<references/>
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==About this Structure==
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__TOC__
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[[3e1h]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Neurospora_crassa Neurospora crassa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3E1H OCA].
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</StructureSection>
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==Reference==
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<ref group="xtra">PMID:018462950</ref><references group="xtra"/>
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[[Category: Naringenin-chalcone synthase]]
[[Category: Naringenin-chalcone synthase]]
[[Category: Neurospora crassa]]
[[Category: Neurospora crassa]]
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[[Category: Goyal, A.]]
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[[Category: Goyal, A]]
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[[Category: Rahman, A.]]
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[[Category: Rahman, A]]
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[[Category: Sankaranarayanan, R.]]
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[[Category: Sankaranarayanan, R]]
[[Category: Acyltransferase]]
[[Category: Acyltransferase]]
[[Category: Resorcinolic lipid synthase]]
[[Category: Resorcinolic lipid synthase]]
[[Category: Transferase]]
[[Category: Transferase]]
[[Category: Type iii pk]]
[[Category: Type iii pk]]

Revision as of 13:29, 19 November 2014

Crystal structure of a type III polyketide synthase PKSIIINc from Neurospora crassa

3e1h, resolution 2.58Å

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