This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
2x1q
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | [[ | + | ==GELSOLIN NANOBODY== |
| + | <StructureSection load='2x1q' size='340' side='right' caption='[[2x1q]], [[Resolution|resolution]] 1.06Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[2x1q]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lama_glama Lama glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X1Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2X1Q FirstGlance]. <br> | ||
| + | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2x1p|2x1p]], [[2x1o|2x1o]]</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2x1q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2x1q OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2x1q RCSB], [http://www.ebi.ac.uk/pdbsum/2x1q PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | RNA interference has tremendously advanced our understanding of gene function but recent reports have exposed undesirable side-effects. Recombinant Camelid single-domain antibodies (VHHs) provide an attractive means for studying protein function without affecting gene expression. We raised VHHs against gelsolin (GsnVHHs), a multifunctional actin-binding protein that controls cellular actin organization and migration. GsnVHH-induced delocalization of gelsolin to mitochondria or the nucleus in mammalian cells reveals distinct subpopulations including free gelsolin and actin-bound gelsolin complexes. GsnVHH 13 specifically recognizes Ca(2+)-activated gelsolin (K (d) approximately 10 nM) while GsnVHH 11 binds gelsolin irrespective of Ca(2+) (K (d) approximately 5 nM) but completely blocks its interaction with G-actin. Both GsnVHHs trace gelsolin in membrane ruffles of EGF-stimulated MCF-7 cells and delay cell migration without affecting F-actin severing/capping or actin nucleation activities by gelsolin. We conclude that VHHs represent a potent way of blocking structural proteins and that actin nucleation by gelsolin is more complex than previously anticipated. | ||
| - | + | A llama-derived gelsolin single-domain antibody blocks gelsolin-G-actin interaction.,Van den Abbeele A, De Clercq S, De Ganck A, De Corte V, Van Loo B, Soror SH, Srinivasan V, Steyaert J, Vandekerckhove J, Gettemans J Cell Mol Life Sci. 2010 May;67(9):1519-35. Epub 2010 Feb 7. PMID:20140750<ref>PMID:20140750</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| + | </div> | ||
| - | + | ==See Also== | |
| - | + | *[[Gelsolin|Gelsolin]] | |
| - | == | + | == References == |
| - | [[ | + | <references/> |
| - | + | __TOC__ | |
| - | == | + | </StructureSection> |
| - | < | + | |
[[Category: Lama glama]] | [[Category: Lama glama]] | ||
| - | [[Category: Abbeele, A Van Den | + | [[Category: Abbeele, A Van Den]] |
| - | [[Category: Corte, V De | + | [[Category: Corte, V De]] |
| - | [[Category: Declercq, S | + | [[Category: Declercq, S]] |
| - | [[Category: Ganck, A De | + | [[Category: Ganck, A De]] |
| - | [[Category: Gettemans, J | + | [[Category: Gettemans, J]] |
| - | [[Category: Kerckhove, J Van De | + | [[Category: Kerckhove, J Van De]] |
| - | [[Category: Loo, B Van | + | [[Category: Loo, B Van]] |
| - | [[Category: Srinivasan, V | + | [[Category: Srinivasan, V]] |
| - | [[Category: Steyaert, J | + | [[Category: Steyaert, J]] |
[[Category: Contractile protein]] | [[Category: Contractile protein]] | ||
Revision as of 13:35, 19 November 2014
GELSOLIN NANOBODY
| |||||||||||
