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3dxb

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[[Image:3dxb.png|left|200px]]
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==Structure of the UHM domain of Puf60 fused to thioredoxin==
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<StructureSection load='3dxb' size='340' side='right' caption='[[3dxb]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3dxb]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_o157:h7 Escherichia coli o157:h7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DXB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3DXB FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">trxA, Z5291, ECs4714 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83334 Escherichia coli O157:H7])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3dxb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3dxb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3dxb RCSB], [http://www.ebi.ac.uk/pdbsum/3dxb PDBsum]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/dx/3dxb_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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PUF60 is an essential splicing factor functionally related and homologous to U2AF(65). Its C-terminal domain belongs to the family of U2AF (U2 auxiliary factor) homology motifs (UHM), a subgroup of RNA recognition motifs that bind to tryptophan-containing linear peptide motifs (UHM ligand motifs, ULMs) in several nuclear proteins. Here, we show that the Puf60 UHM is mainly monomeric in physiological buffer, whereas its dimerization is induced upon the addition of SDS. The crystal structure of PUF60-UHM at 2.2 angstroms resolution, NMR data, and mutational analysis reveal that the dimer interface is mediated by electrostatic interactions involving a flexible loop. Using glutathione S-transferase pulldown experiments, isothermal titration calorimetry, and NMR titrations, we find that Puf60-UHM binds to ULM sequences in the splicing factors SF1, U2AF65, and SF3b155. Compared with U2AF65-UHM, Puf60-UHM has distinct binding preferences to ULMs in the N terminus of SF3b155. Our data suggest that the functional cooperativity between U2AF65 and Puf60 may involve simultaneous interactions of the two proteins with SF3b155.
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{{STRUCTURE_3dxb| PDB=3dxb | SCENE= }}
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Dimerization and protein binding specificity of the U2AF homology motif of the splicing factor Puf60.,Corsini L, Hothorn M, Stier G, Rybin V, Scheffzek K, Gibson TJ, Sattler M J Biol Chem. 2009 Jan 2;284(1):630-9. Epub 2008 Oct 29. PMID:18974054<ref>PMID:18974054</ref>
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===Structure of the UHM domain of Puf60 fused to thioredoxin===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_18974054}}
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==About this Structure==
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[[3dxb]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_o157:h7 Escherichia coli o157:h7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DXB OCA].
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==See Also==
==See Also==
*[[Thioredoxin|Thioredoxin]]
*[[Thioredoxin|Thioredoxin]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:018974054</ref><ref group="xtra">PMID:018780816</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli o157:h7]]
[[Category: Escherichia coli o157:h7]]
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[[Category: Corsini, L.]]
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[[Category: Corsini, L]]
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[[Category: Hothorn, M.]]
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[[Category: Hothorn, M]]
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[[Category: Sattler, M.]]
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[[Category: Sattler, M]]
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[[Category: Scheffzek, K.]]
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[[Category: Scheffzek, K]]
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[[Category: Stier, G.]]
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[[Category: Stier, G]]
[[Category: Electron transport]]
[[Category: Electron transport]]
[[Category: Fbp interacting repressor]]
[[Category: Fbp interacting repressor]]

Revision as of 13:39, 19 November 2014

Structure of the UHM domain of Puf60 fused to thioredoxin

3dxb, resolution 2.20Å

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