1lom

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[[Image:1lom.gif|left|200px]]<br /><applet load="1lom" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1lom.gif|left|200px]]
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caption="1lom, resolution 1.72&Aring;" />
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'''CYANOVIRIN-N DOUBLE MUTANT P51S S52P'''<br />
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{{Structure
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|PDB= 1lom |SIZE=350|CAPTION= <scene name='initialview01'>1lom</scene>, resolution 1.72&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> and <scene name='pdbligand=CA:CALCIUM ION'>CA</scene>
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|ACTIVITY=
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|GENE=
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}}
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'''CYANOVIRIN-N DOUBLE MUTANT P51S S52P'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1LOM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Nostoc_ellipsosporum Nostoc ellipsosporum] with <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LOM OCA].
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1LOM is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Nostoc_ellipsosporum Nostoc ellipsosporum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LOM OCA].
==Reference==
==Reference==
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Domain-swapped structure of a mutant of cyanovirin-N., Botos I, Mori T, Cartner LK, Boyd MR, Wlodawer A, Biochem Biophys Res Commun. 2002 May 31;294(1):184-90. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12054761 12054761]
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Domain-swapped structure of a mutant of cyanovirin-N., Botos I, Mori T, Cartner LK, Boyd MR, Wlodawer A, Biochem Biophys Res Commun. 2002 May 31;294(1):184-90. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12054761 12054761]
[[Category: Nostoc ellipsosporum]]
[[Category: Nostoc ellipsosporum]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: hiv-inactivating]]
[[Category: hiv-inactivating]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:46:53 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:33:17 2008''

Revision as of 10:33, 20 March 2008

Image:1lom.gif


PDB ID 1lom

Drag the structure with the mouse to rotate
, resolution 1.72Å
Ligands: and
Coordinates: save as pdb, mmCIF, xml



CYANOVIRIN-N DOUBLE MUTANT P51S S52P


Overview

Cyanovirin-N (CV-N) is a potent 11 kDa HIV-inactivating protein that binds with high affinity to the HIV surface envelope protein gp120. A double mutant P51S/S52P of CV-N was engineered by swapping two critical hinge-region residues Pro51 and Ser52. This mutant has biochemical and biophysical characteristics equivalent to the wild-type CV-N and its structure resembles that of wild-type CV-N. However, the mutant shows a different orientation in the hinge region that connects two domains of the protein. The observation that this double mutant crystallizes under a wide variety of conditions challenges some of the current hypotheses on domain swapping and on the role of hinge-region proline residues in domain orientation. The current structure contributes to the understanding of domain swapping in cyanovirins, permitting rational design of domain-swapped CV-N mutants.

About this Structure

1LOM is a Single protein structure of sequence from Nostoc ellipsosporum. Full crystallographic information is available from OCA.

Reference

Domain-swapped structure of a mutant of cyanovirin-N., Botos I, Mori T, Cartner LK, Boyd MR, Wlodawer A, Biochem Biophys Res Commun. 2002 May 31;294(1):184-90. PMID:12054761

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