4i68

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{{STRUCTURE_4i68| PDB=4i68 | SCENE= }}
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==Crystal structure of the R444A / R449A double mutant of the HERA RNA helicase RRM domain==
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===Crystal structure of the R444A / R449A double mutant of the HERA RNA helicase RRM domain===
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<StructureSection load='4i68' size='340' side='right' caption='[[4i68]], [[Resolution|resolution]] 1.63&Aring;' scene=''>
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{{ABSTRACT_PUBMED_23625962}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4i68]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"flavobacterium_thermophilum"_yoshida_and_oshima_1971 "flavobacterium thermophilum" yoshida and oshima 1971]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4I68 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4I68 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3i31|3i31]], [[4i67|4i67]], [[4i69|4i69]]</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">TT_C1895 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=274 "Flavobacterium thermophilum" Yoshida and Oshima 1971])</td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/RNA_helicase RNA helicase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.13 3.6.4.13] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4i68 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4i68 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4i68 RCSB], [http://www.ebi.ac.uk/pdbsum/4i68 PDBsum]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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DEAD box helicases catalyze the ATP-dependent destabilization of RNA duplexes. Whereas duplex separation is mediated by the helicase core shared by all members of the family, flanking domains often contribute to binding of the RNA substrate. The Thermus thermophilus DEAD-box helicase Hera (for "heat-resistant RNA-binding ATPase") contains a C-terminal RNA-binding domain (RBD). We have analyzed RNA binding to the Hera RBD by a combination of mutational analyses, nuclear magnetic resonance and X-ray crystallography, and identify residues on helix alpha1 and the C-terminus as the main determinants for high-affinity RNA binding. A crystal structure of the RBD in complex with a single-stranded RNA resolves the RNA-protein interactions in the RBD core region around helix alpha1. Differences in RNA binding to the Hera RBD and to the structurally similar RBD of the Bacillus subtilis DEAD box helicase YxiN illustrate the versatility of RNA recognition motifs as RNA-binding platforms. Comparison of chemical shift perturbation patterns elicited by different RNAs, and the effect of sequence changes in the RNA on binding and unwinding show that the RBD binds a single-stranded RNA region at the core and simultaneously contacts double-stranded RNA through its C-terminal tail. The helicase core then unwinds an adjacent RNA duplex. Overall, the mode of RNA binding by Hera is consistent with a possible function as a general RNA chaperone.
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==About this Structure==
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Recognition of two distinct elements in the RNA substrate by the RNA-binding domain of the T. thermophilus DEAD box helicase Hera.,Steimer L, Wurm JP, Linden MH, Rudolph MG, Wohnert J, Klostermeier D Nucleic Acids Res. 2013 Apr 25. PMID:23625962<ref>PMID:23625962</ref>
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[[4i68]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4I68 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<ref group="xtra">PMID:023625962</ref><references group="xtra"/><references/>
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</div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Flavobacterium thermophilum yoshida and oshima 1971]]
[[Category: RNA helicase]]
[[Category: RNA helicase]]
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[[Category: Thermus thermophilus]]
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[[Category: Klostermeier, D]]
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[[Category: Klostermeier, D.]]
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[[Category: Rudolph, M G]]
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[[Category: Rudolph, M G.]]
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[[Category: Atp binding]]
[[Category: Atp binding]]
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[[Category: Dead box protein]]
[[Category: Hydrolase]]
[[Category: Hydrolase]]
[[Category: Nucleotide binding]]
[[Category: Nucleotide binding]]
[[Category: Rna binding protein]]
[[Category: Rna binding protein]]
[[Category: Rna recognition motif]]
[[Category: Rna recognition motif]]

Revision as of 06:04, 26 November 2014

Crystal structure of the R444A / R449A double mutant of the HERA RNA helicase RRM domain

4i68, resolution 1.63Å

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