1lsu

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1lsu.gif|left|200px]]<br /><applet load="1lsu" size="350" color="white" frame="true" align="right" spinBox="true"
+
[[Image:1lsu.gif|left|200px]]
-
caption="1lsu, resolution 2.85&Aring;" />
+
 
-
'''KTN Bsu222 Crystal Structure in Complex with NADH'''<br />
+
{{Structure
 +
|PDB= 1lsu |SIZE=350|CAPTION= <scene name='initialview01'>1lsu</scene>, resolution 2.85&Aring;
 +
|SITE=
 +
|LIGAND= <scene name='pdbligand=NAI:1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE'>NAI</scene>
 +
|ACTIVITY=
 +
|GENE= KtrA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis])
 +
}}
 +
 
 +
'''KTN Bsu222 Crystal Structure in Complex with NADH'''
 +
 
==Overview==
==Overview==
Line 7: Line 16:
==About this Structure==
==About this Structure==
-
1LSU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis] with <scene name='pdbligand=NAI:'>NAI</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LSU OCA].
+
1LSU is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LSU OCA].
==Reference==
==Reference==
-
A mechanism of regulating transmembrane potassium flux through a ligand-mediated conformational switch., Roosild TP, Miller S, Booth IR, Choe S, Cell. 2002 Jun 14;109(6):781-91. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12086676 12086676]
+
A mechanism of regulating transmembrane potassium flux through a ligand-mediated conformational switch., Roosild TP, Miller S, Booth IR, Choe S, Cell. 2002 Jun 14;109(6):781-91. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12086676 12086676]
[[Category: Bacillus subtilis]]
[[Category: Bacillus subtilis]]
[[Category: Single protein]]
[[Category: Single protein]]
Line 26: Line 35:
[[Category: rossman fold]]
[[Category: rossman fold]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:48:06 2008''
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:34:48 2008''

Revision as of 10:34, 20 March 2008

Image:1lsu.gif


PDB ID 1lsu

Drag the structure with the mouse to rotate
, resolution 2.85Å
Ligands:
Gene: KtrA (Bacillus subtilis)
Coordinates: save as pdb, mmCIF, xml



KTN Bsu222 Crystal Structure in Complex with NADH


Overview

The regulation of cation content is critical for cell growth. However, the molecular mechanisms that gate the systems that control K+ movements remain unclear. KTN is a highly conserved cytoplasmic domain present ubiquitously in a variety of prokaryotic and eukaryotic K+ channels and transporters. Here we report crystal structures for two representative KTN domains that reveal a dimeric hinged assembly. Alternative ligands NAD+ and NADH block or vacate, respectively, the hinge region affecting the dimer's conformational flexibility. Conserved, surface-exposed hydrophobic patches that become coplanar upon hinge closure provide an assembly interface for KTN tetramerization. Mutational analysis using the KefC system demonstrates that this domain directly interacts with its respective transmembrane constituent, coupling ligand-mediated KTN conformational changes to the permease's activity.

About this Structure

1LSU is a Single protein structure of sequence from Bacillus subtilis. Full crystallographic information is available from OCA.

Reference

A mechanism of regulating transmembrane potassium flux through a ligand-mediated conformational switch., Roosild TP, Miller S, Booth IR, Choe S, Cell. 2002 Jun 14;109(6):781-91. PMID:12086676

Page seeded by OCA on Thu Mar 20 12:34:48 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1lsu"
Personal tools