4v0k
From Proteopedia
(Difference between revisions)
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4v0k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4v0k OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4v0k RCSB], [http://www.ebi.ac.uk/pdbsum/4v0k PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4v0k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4v0k OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4v0k RCSB], [http://www.ebi.ac.uk/pdbsum/4v0k PDBsum]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The BBSome is a coat-like ciliary trafficking complex composed of proteins mutated in Bardet-Biedl syndrome (BBS). A critical step in BBSome-mediated sorting is recruitment of the BBSome to membranes by the GTP-bound Arf-like GTPase ARL6. We have determined crystal structures of Chlamydomonas reinhardtii ARL6-GDP, ARL6-GTP and the ARL6-GTP-BBS1 complex. The structures demonstrate how ARL6-GTP binds the BBS1 beta-propeller at blades 1 and 7 and explain why GTP- but not GDP-bound ARL6 can recruit the BBSome to membranes. Single point mutations in the ARL6-GTP-BBS1 interface abolish the interaction of ARL6 with the BBSome and prevent the import of BBSomes into cilia. Furthermore, we show that BBS1 with the M390R mutation, responsible for 30% of all reported BBS disease cases, fails to interact with ARL6-GTP, thus providing a molecular rationale for patient pathologies. | ||
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| + | Structural basis for membrane targeting of the BBSome by ARL6.,Mourao A, Nager AR, Nachury MV, Lorentzen E Nat Struct Mol Biol. 2014 Nov 17. doi: 10.1038/nsmb.2920. PMID:25402481<ref>PMID:25402481</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Revision as of 06:09, 26 November 2014
Crystal structure of the CrARL6DN in the GDP bound form
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