1lta
From Proteopedia
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| - | [[Image:1lta.jpg|left|200px]] | + | [[Image:1lta.jpg|left|200px]] |
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| - | '''2.2 ANGSTROMS CRYSTAL STRUCTURE OF E. COLI HEAT-LABILE ENTEROTOXIN (LT) WITH BOUND GALACTOSE''' | + | {{Structure |
| + | |PDB= 1lta |SIZE=350|CAPTION= <scene name='initialview01'>1lta</scene>, resolution 2.2Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''2.2 ANGSTROMS CRYSTAL STRUCTURE OF E. COLI HEAT-LABILE ENTEROTOXIN (LT) WITH BOUND GALACTOSE''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1LTA is a [ | + | 1LTA is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LTA OCA]. |
==Reference== | ==Reference== | ||
| - | Galactose-binding site in Escherichia coli heat-labile enterotoxin (LT) and cholera toxin (CT)., Merritt EA, Sixma TK, Kalk KH, van Zanten BA, Hol WG, Mol Microbiol. 1994 Aug;13(4):745-53. PMID:[http:// | + | Galactose-binding site in Escherichia coli heat-labile enterotoxin (LT) and cholera toxin (CT)., Merritt EA, Sixma TK, Kalk KH, van Zanten BA, Hol WG, Mol Microbiol. 1994 Aug;13(4):745-53. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7997185 7997185] |
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: enterotoxin]] | [[Category: enterotoxin]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:34:55 2008'' |
Revision as of 10:34, 20 March 2008
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
2.2 ANGSTROMS CRYSTAL STRUCTURE OF E. COLI HEAT-LABILE ENTEROTOXIN (LT) WITH BOUND GALACTOSE
Overview
The galactose-binding site in cholera toxin and the closely related heat-labile enterotoxin (LT) from Escherichia coli is an attractive target for the rational design of potential anti-cholera drugs. In this paper we analyse the molecular structure of this binding site as seen in several crystal structures, including that of an LT:galactose complex which we report here at 2.2 A resolution. The binding surface on the free toxin contains several tightly associated water molecules and a relatively flexible loop consisting of residues 51-60 of the B subunit. During receptor binding this loop becomes tightly ordered by forming hydrogen bonds jointly to the GM1 pentasaccharide and to a set of water molecules which stabilize the toxin:receptor complex.
About this Structure
1LTA is a Protein complex structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Galactose-binding site in Escherichia coli heat-labile enterotoxin (LT) and cholera toxin (CT)., Merritt EA, Sixma TK, Kalk KH, van Zanten BA, Hol WG, Mol Microbiol. 1994 Aug;13(4):745-53. PMID:7997185
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