1lvr
From Proteopedia
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| - | [[Image:1lvr.gif|left|200px]] | + | [[Image:1lvr.gif|left|200px]] |
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| - | '''IC3 of CB1 (L431A,A432L) Bound to G(alpha)i''' | + | {{Structure |
| + | |PDB= 1lvr |SIZE=350|CAPTION= <scene name='initialview01'>1lvr</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
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| + | '''IC3 of CB1 (L431A,A432L) Bound to G(alpha)i''' | ||
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==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1LVR is a [ | + | 1LVR is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LVR OCA]. |
==Reference== | ==Reference== | ||
| - | Cannabinoid receptor-G protein interactions: G(alphai1)-bound structures of IC3 and a mutant with altered G protein specificity., Ulfers AL, McMurry JL, Miller A, Wang L, Kendall DA, Mierke DF, Protein Sci. 2002 Oct;11(10):2526-31. PMID:[http:// | + | Cannabinoid receptor-G protein interactions: G(alphai1)-bound structures of IC3 and a mutant with altered G protein specificity., Ulfers AL, McMurry JL, Miller A, Wang L, Kendall DA, Mierke DF, Protein Sci. 2002 Oct;11(10):2526-31. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12237474 12237474] |
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Kendall, D A.]] | [[Category: Kendall, D A.]] | ||
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[[Category: cannabinoid 1 receptor (cb1)]] | [[Category: cannabinoid 1 receptor (cb1)]] | ||
[[Category: intracellular loop 3 (ic3)]] | [[Category: intracellular loop 3 (ic3)]] | ||
| - | [[Category: transferred | + | [[Category: transferred no]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:35:46 2008'' |
Revision as of 10:35, 20 March 2008
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
IC3 of CB1 (L431A,A432L) Bound to G(alpha)i
Overview
The structure of the C-terminal region of the third cytoplasmic loop (IC3) of the cannabinoid receptor one (CB1) bound to G(alphai1) has been determined using transferred nuclear Overhauser effects (NOEs). The wild-type IC3 sequence is helical when associated with G(alphai1). In contrast, a peptide containing the amino-acid inversion, Ala(341)-Leu(342) adopts a single turn. These findings correlate with the attenuated G(i) association of CB1 with the Ala(341)-Leu(342) mutation previously observed in vivo and the diminished stimulation of G(alphai1) GTPase activity by the corresponding peptide demonstrated in vitro here. These results, the first to report the structure of a GPCR domain while associated with G protein, imply the C-terminus of CB1 IC3, a region with high-sequence conservation among G-protein coupled receptors, must be helical for efficient coupling and activation of the G(i) protein.
About this Structure
1LVR is a Single protein structure of sequence from [1]. Full crystallographic information is available from OCA.
Reference
Cannabinoid receptor-G protein interactions: G(alphai1)-bound structures of IC3 and a mutant with altered G protein specificity., Ulfers AL, McMurry JL, Miller A, Wang L, Kendall DA, Mierke DF, Protein Sci. 2002 Oct;11(10):2526-31. PMID:12237474
Page seeded by OCA on Thu Mar 20 12:35:46 2008
