1lz9
From Proteopedia
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| - | [[Image:1lz9.gif|left|200px]] | + | [[Image:1lz9.gif|left|200px]] |
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| - | '''ANOMALOUS SIGNAL OF SOLVENT BROMINES USED FOR PHASING OF LYSOZYME''' | + | {{Structure |
| + | |PDB= 1lz9 |SIZE=350|CAPTION= <scene name='initialview01'>1lz9</scene>, resolution 1.70Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=BR:BROMIDE+ION'>BR</scene> and <scene name='pdbligand=NA:SODIUM ION'>NA</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''ANOMALOUS SIGNAL OF SOLVENT BROMINES USED FOR PHASING OF LYSOZYME''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1LZ9 is a [ | + | 1LZ9 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LZ9 OCA]. |
==Reference== | ==Reference== | ||
| - | Anomalous signal of solvent bromides used for phasing of lysozyme., Dauter Z, Dauter M, J Mol Biol. 1999 May 28;289(1):93-101. PMID:[http:// | + | Anomalous signal of solvent bromides used for phasing of lysozyme., Dauter Z, Dauter M, J Mol Biol. 1999 May 28;289(1):93-101. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10339408 10339408] |
[[Category: Gallus gallus]] | [[Category: Gallus gallus]] | ||
[[Category: Lysozyme]] | [[Category: Lysozyme]] | ||
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[[Category: lysozyme]] | [[Category: lysozyme]] | ||
[[Category: single wavelength]] | [[Category: single wavelength]] | ||
| - | [[Category: solvent | + | [[Category: solvent bromide]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:36:58 2008'' |
Revision as of 10:36, 20 March 2008
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| , resolution 1.70Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Activity: | Lysozyme, with EC number 3.2.1.17 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
ANOMALOUS SIGNAL OF SOLVENT BROMINES USED FOR PHASING OF LYSOZYME
Overview
The anomalous signal of bromide ions, present in the crystal structure of tetragonal hen egg-white lysozyme through the substitution of NaCl by NaBr in the crystallization medium, was used for phasing of X-ray data collected to 1.7 A resolution with a wavelength near the absorption edge of bromine. Phasing of a single wavelength data set, based purely on anomalous deltaf " contribution, led to easily interpretable electron density, equivalent to the complete multiwavelength anonalous dispersion phasing based on four-wavelength data. The classic small-structure direct methods program SHELXS run against all anomalous differences gave a successful solution of six highest peaks corresponding to six bromide ions in the structure with data limited up to a resolution of 3.5 A. Interpretable maps were obtained at a resolution up to 3.0 A using programs MLPHARE and DM. Bromide ions occupy well ordered positions at the protein surface. Phasing based on the single wavelength signal of anomalous scatterers introduced into the ordered solvent shell can be proposed as a tool for solving structures of well diffracting crystals.
About this Structure
1LZ9 is a Single protein structure of sequence from Gallus gallus. Full crystallographic information is available from OCA.
Reference
Anomalous signal of solvent bromides used for phasing of lysozyme., Dauter Z, Dauter M, J Mol Biol. 1999 May 28;289(1):93-101. PMID:10339408
Page seeded by OCA on Thu Mar 20 12:36:58 2008
