3f9v

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[[Image:3f9v.png|left|200px]]
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==Crystal Structure Of A Near Full-Length Archaeal MCM: Functional Insights For An AAA+ Hexameric Helicase==
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<StructureSection load='3f9v' size='340' side='right' caption='[[3f9v]], [[Resolution|resolution]] 4.35&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3f9v]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3F9V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3F9V FirstGlance]. <br>
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</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MCM, SSO0774 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2287 Sulfolobus solfataricus])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3f9v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3f9v OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3f9v RCSB], [http://www.ebi.ac.uk/pdbsum/3f9v PDBsum]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f9/3f9v_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The minichromosome maintenance protein (MCM) complex is an essential replicative helicase for DNA replication in Archaea and Eukaryotes. Whereas the eukaryotic complex consists of 6 homologous proteins (MCM2-7), the archaeon Sulfolobus solfataricus has only 1 MCM protein (ssoMCM), 6 subunits of which form a homohexamer. Here, we report a 4.35-A crystal structure of the near-full-length ssoMCM. The structure shows an elongated fold, with 5 subdomains that are organized into 2 large N- and C-terminal domains. A near-full-length ssoMCM hexamer generated based on the 6-fold symmetry of the N-terminal Methanothermobacter thermautotrophicus (mtMCM) hexamer shows intersubunit distances suitable for bonding contacts, including the interface around the ATP pocket. Four unusual beta-hairpins of each subunit are located inside the central channel or around the side channels in the hexamer. Additionally, the hexamer fits well into the double-hexamer EM map of mtMCM. Our mutational analysis of residues at the intersubunit interfaces and around the side channels demonstrates their critical roles for hexamerization and helicase function. These structural and biochemical results provide a basis for future study of the helicase mechanisms of the archaeal and eukaryotic MCM complexes in DNA replication.
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{{STRUCTURE_3f9v| PDB=3f9v | SCENE= }}
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Crystal structure of a near-full-length archaeal MCM: Functional insights for an AAA+ hexameric helicase.,Brewster AS, Wang G, Yu X, Greenleaf WB, Carazo JM, Tjajadia M, Klein MG, Chen XS Proc Natl Acad Sci U S A. 2008 Dec 23;105(51):20191-6. Epub 2008 Dec 10. PMID:19073923<ref>PMID:19073923</ref>
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===Crystal Structure Of A Near Full-Length Archaeal MCM: Functional Insights For An AAA+ Hexameric Helicase===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_19073923}}
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== References ==
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<references/>
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==About this Structure==
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__TOC__
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[[3f9v]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3F9V OCA].
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</StructureSection>
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==Reference==
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<ref group="xtra">PMID:019073923</ref><references group="xtra"/>
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[[Category: Sulfolobus solfataricus]]
[[Category: Sulfolobus solfataricus]]
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[[Category: Brewster, A S.]]
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[[Category: Brewster, A S]]
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[[Category: Chen, X J.]]
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[[Category: Chen, X J]]
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[[Category: Greenleaf, W.]]
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[[Category: Greenleaf, W]]
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[[Category: Klein, M.]]
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[[Category: Klein, M]]
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[[Category: Tjajadi, M.]]
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[[Category: Tjajadi, M]]
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[[Category: Wang, G G.]]
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[[Category: Wang, G G]]
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[[Category: Yu, X.]]
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[[Category: Yu, X]]
[[Category: Aaa+ protein]]
[[Category: Aaa+ protein]]
[[Category: Atp-binding]]
[[Category: Atp-binding]]

Revision as of 08:32, 26 November 2014

Crystal Structure Of A Near Full-Length Archaeal MCM: Functional Insights For An AAA+ Hexameric Helicase

3f9v, resolution 4.35Å

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