1lzt
From Proteopedia
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| - | [[Image:1lzt.gif|left|200px]] | + | [[Image:1lzt.gif|left|200px]] |
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| - | '''REFINEMENT OF TRICLINIC LYSOZYME''' | + | {{Structure |
| + | |PDB= 1lzt |SIZE=350|CAPTION= <scene name='initialview01'>1lzt</scene>, resolution 1.97Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''REFINEMENT OF TRICLINIC LYSOZYME''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1LZT is a [ | + | 1LZT is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LZT OCA]. |
==Reference== | ==Reference== | ||
| - | Refinement of triclinic lysozyme: I. Fourier and least-squares methods., Hodsdon JM, Brown GM, Sieker LC, Jensen LH, Acta Crystallogr B. 1990 Feb 1;46 ( Pt 1):54-62. PMID:[http:// | + | Refinement of triclinic lysozyme: I. Fourier and least-squares methods., Hodsdon JM, Brown GM, Sieker LC, Jensen LH, Acta Crystallogr B. 1990 Feb 1;46 ( Pt 1):54-62. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/2302326 2302326] |
[[Category: Gallus gallus]] | [[Category: Gallus gallus]] | ||
[[Category: Lysozyme]] | [[Category: Lysozyme]] | ||
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[[Category: hydrolase(o-glycosyl)]] | [[Category: hydrolase(o-glycosyl)]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:37:06 2008'' |
Revision as of 10:37, 20 March 2008
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| , resolution 1.97Å | |||||||
|---|---|---|---|---|---|---|---|
| Activity: | Lysozyme, with EC number 3.2.1.17 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
REFINEMENT OF TRICLINIC LYSOZYME
Overview
X-ray diffraction data to 1.5 A resolution have been collected for triclinic crystals of hen egg white lysozyme. The triclinic model was derived from the tetragonal one by the rotation function and refined initially by Fo-Fc and differential difference syntheses against 2 A resolution data. Refinement was continued by differential difference cycles against the 1.5 A data until R was reduced to 0.220. Although the initial refinement was rapid, it was subsequently a matter of attrition, leading to a complete recheck of the data and the discovery of systematic error which affected primarily the high-resolution data. Refinement was continued against the corrected 2 A data by block-diagonal least squares. After five cycles the refinement was terminated at R = 0.254 because of the imminent availability of a preferred refinement program. Problems with the protein model, the solvent, and the interaction of the scale and thermal parameters are discussed. The experiences gained in this study are summarized.
About this Structure
1LZT is a Single protein structure of sequence from Gallus gallus. Full crystallographic information is available from OCA.
Reference
Refinement of triclinic lysozyme: I. Fourier and least-squares methods., Hodsdon JM, Brown GM, Sieker LC, Jensen LH, Acta Crystallogr B. 1990 Feb 1;46 ( Pt 1):54-62. PMID:2302326
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