3exs

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[[Image:3exs.png|left|200px]]
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==Crystal structure of KGPDC from Streptococcus mutans in complex with D-R5P==
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<StructureSection load='3exs' size='340' side='right' caption='[[3exs]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3exs]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_mutans Streptococcus mutans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EXS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3EXS FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=5RP:RIBULOSE-5-PHOSPHATE'>5RP</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3exr|3exr]], [[3ext|3ext]]</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ulaD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1309 Streptococcus mutans])</td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/3-dehydro-L-gulonate-6-phosphate_decarboxylase 3-dehydro-L-gulonate-6-phosphate decarboxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.1.85 4.1.1.85] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3exs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3exs OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3exs RCSB], [http://www.ebi.ac.uk/pdbsum/3exs PDBsum]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ex/3exs_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The 3-keto-L-gulonate 6-phosphate decarboxylase (KGPDC) catalyses the decarboxylation of 3-keto-L-gulonate 6-phosphate to L-xylulose in the presence of magnesium ions. The enzyme is involved in L-ascorbate metabolism and plays an essential role in the pathway of glucuronate interconversion. Crystal structures of Streptococcus mutans KGPDC were determined in the absence and presence of the product analog D-ribulose 5-phosphate. We have observed an 8 A alphaB-helix movement and other structural rearrangements around the active site between the apo-structures and product analog bound structure. These drastic conformational changes upon ligand binding are the first observation of this kind for the KGPDC family. The flexibilities of both the alpha-helix lid and the side chains of Arg144 and Arg197 are associated with substrate binding and product releasing. The open-closed conformational changes of the active site, through the movements of the alpha-helix lid and the arginine residues are important for substrate binding and catalysis.
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{{STRUCTURE_3exs| PDB=3exs | SCENE= }}
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Open-closed conformational change revealed by the crystal structures of 3-keto-L-gulonate 6-phosphate decarboxylase from Streptococcus mutans.,Li GL, Liu X, Nan J, Brostromer E, Li LF, Su XD Biochem Biophys Res Commun. 2009 Apr 10;381(3):429-33. Epub 2009 Feb 15. PMID:19222992<ref>PMID:19222992</ref>
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===Crystal structure of KGPDC from Streptococcus mutans in complex with D-R5P===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_19222992}}
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== References ==
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<references/>
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==About this Structure==
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__TOC__
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[[3exs]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_mutans Streptococcus mutans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EXS OCA].
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</StructureSection>
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==Reference==
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<ref group="xtra">PMID:019222992</ref><references group="xtra"/>
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[[Category: 3-dehydro-L-gulonate-6-phosphate decarboxylase]]
[[Category: 3-dehydro-L-gulonate-6-phosphate decarboxylase]]
[[Category: Streptococcus mutans]]
[[Category: Streptococcus mutans]]
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[[Category: Li, G L.]]
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[[Category: Li, G L]]
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[[Category: Li, L F.]]
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[[Category: Li, L F]]
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[[Category: Liu, X.]]
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[[Category: Liu, X]]
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[[Category: Su, X D.]]
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[[Category: Su, X D]]
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[[Category: Wang, K T.]]
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[[Category: Wang, K T]]
[[Category: Beta barrel]]
[[Category: Beta barrel]]
[[Category: Lyase]]
[[Category: Lyase]]

Revision as of 08:36, 26 November 2014

Crystal structure of KGPDC from Streptococcus mutans in complex with D-R5P

3exs, resolution 2.50Å

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