3f1j

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[[Image:3f1j.png|left|200px]]
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==Crystal structure of the Borna disease virus matrix protein (BDV-M) reveals RNA binding properties==
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<StructureSection load='3f1j' size='340' side='right' caption='[[3f1j]], [[Resolution|resolution]] 2.65&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3f1j]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Borna_disease_virus Borna disease virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3F1J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3F1J FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=C5P:CYTIDINE-5-MONOPHOSPHATE'>C5P</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">M ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=12455 Borna disease virus])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3f1j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3f1j OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3f1j RCSB], [http://www.ebi.ac.uk/pdbsum/3f1j PDBsum]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f1/3f1j_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Borna disease virus (BDV) is a neurotropic enveloped RNA virus that causes a noncytolytic, persistent infection of the central nervous system in mammals. BDV belongs to the order Mononegavirales, which also includes the negative-strand RNA viruses (NSVs) Ebola, Marburg, vesicular stomatitis, rabies, mumps, and measles. BDV-M, the matrix protein (M-protein) of BDV, is the smallest M-protein (16.2 kDa) among the NSVs. M-proteins play a critical role in virus assembly and budding, mediating the interaction between the viral capsid, envelope, and glycoprotein spikes, and are as such responsible for the structural stability and individual form of virus particles. Here, we report the 3D structure of BDV-M, a full-length M-protein structure from a nonsegmented RNA NSV. The BDV-M monomer exhibits structural similarity to the N-terminal domain of the Ebola M-protein (VP40), while the surface charge of the tetramer provides clues to the membrane association of BDV-M. Additional electron density in the crystal reveals the presence of bound nucleic acid, interpreted as cytidine-5'-monophosphate. The heterologously expressed BDV-M copurifies with and protects ssRNA oligonucleotides of a median length of 16 nt taken up from the expression host. The results presented here show that BDV-M would be able to bind RNA and lipid membranes simultaneously, expanding the repertoire of M-protein functionalities.
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{{STRUCTURE_3f1j| PDB=3f1j | SCENE= }}
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Crystal structure of the Borna disease virus matrix protein (BDV-M) reveals ssRNA binding properties.,Neumann P, Lieber D, Meyer S, Dautel P, Kerth A, Kraus I, Garten W, Stubbs MT Proc Natl Acad Sci U S A. 2009 Mar 10;106(10):3710-5. Epub 2009 Feb 23. PMID:19237566<ref>PMID:19237566</ref>
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===Crystal structure of the Borna disease virus matrix protein (BDV-M) reveals RNA binding properties===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_19237566}}
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== References ==
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<references/>
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==About this Structure==
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__TOC__
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[[3f1j]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Borna_disease_virus Borna disease virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3F1J OCA].
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</StructureSection>
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==Reference==
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<ref group="xtra">PMID:019237566</ref><references group="xtra"/>
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[[Category: Borna disease virus]]
[[Category: Borna disease virus]]
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[[Category: Dautel, P.]]
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[[Category: Dautel, P]]
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[[Category: Garten, W.]]
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[[Category: Garten, W]]
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[[Category: Kerth, A.]]
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[[Category: Kerth, A]]
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[[Category: Kraus, I.]]
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[[Category: Kraus, I]]
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[[Category: Lieber, D.]]
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[[Category: Lieber, D]]
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[[Category: Meyer, S.]]
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[[Category: Meyer, S]]
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[[Category: Neumann, P.]]
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[[Category: Neumann, P]]
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[[Category: Stubbs, M T.]]
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[[Category: Stubbs, M T]]
[[Category: Bornaviridae]]
[[Category: Bornaviridae]]
[[Category: Bornavirus]]
[[Category: Bornavirus]]

Revision as of 08:37, 26 November 2014

Crystal structure of the Borna disease virus matrix protein (BDV-M) reveals RNA binding properties

3f1j, resolution 2.65Å

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