1m2c
From Proteopedia
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| - | [[Image:1m2c.jpg|left|200px]] | + | [[Image:1m2c.jpg|left|200px]] |
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| - | '''THREE-DIMENSIONAL STRUCTURE OF ALPHA-CONOTOXIN MII, NMR, 14 STRUCTURES''' | + | {{Structure |
| + | |PDB= 1m2c |SIZE=350|CAPTION= <scene name='initialview01'>1m2c</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=NH2:AMINO GROUP'>NH2</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''THREE-DIMENSIONAL STRUCTURE OF ALPHA-CONOTOXIN MII, NMR, 14 STRUCTURES''' | ||
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==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1M2C is a [ | + | 1M2C is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Conus_magus Conus magus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M2C OCA]. |
==Reference== | ==Reference== | ||
| - | A new alpha-conotoxin which targets alpha3beta2 nicotinic acetylcholine receptors., Cartier GE, Yoshikami D, Gray WR, Luo S, Olivera BM, McIntosh JM, J Biol Chem. 1996 Mar 29;271(13):7522-8. PMID:[http:// | + | A new alpha-conotoxin which targets alpha3beta2 nicotinic acetylcholine receptors., Cartier GE, Yoshikami D, Gray WR, Luo S, Olivera BM, McIntosh JM, J Biol Chem. 1996 Mar 29;271(13):7522-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8631783 8631783] |
[[Category: Conus magus]] | [[Category: Conus magus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: subtype specific ligand]] | [[Category: subtype specific ligand]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:38:01 2008'' |
Revision as of 10:38, 20 March 2008
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
THREE-DIMENSIONAL STRUCTURE OF ALPHA-CONOTOXIN MII, NMR, 14 STRUCTURES
Overview
We have isolated a 16-amino acid peptide from the venom of the marine snail Conus magus which potently blocks nicotinic acetylcholine receptors (nAChRs) composed of alpha3beta2 subunits. This peptide, named alpha-conotoxin MII, was identified by electrophysiologically screening venom fractions against cloned nicotinic receptors expressed in Xenopus oocytes. The peptide's structure, which has been confirmed by mass spectrometry and total chemical synthesis, differs significantly from those of all previously isolated alpha-conotoxins. Disulfide bridging, however, is conserved. The toxin blocks the response to acetylcholine in oocytes expressing alpha3beta2 nAChRs with an IC50 of 0.5 nM and is 2-4 orders of magnitude less potent on other nAChR subunit combinations. We have recently reported the isolation and characterization of alpha-conotoxin ImI, which selectively targets homomeric alpha7 neuronal nAChRs. Yet other alpha-conotoxins selectively block the muscle subtype of nAChR. Thus, it is increasingly apparent that alpha-conotoxins represent a significant resource for ligands with which to probe structure-function relationships of various nAChR subtypes.
About this Structure
1M2C is a Single protein structure of sequence from Conus magus. Full crystallographic information is available from OCA.
Reference
A new alpha-conotoxin which targets alpha3beta2 nicotinic acetylcholine receptors., Cartier GE, Yoshikami D, Gray WR, Luo S, Olivera BM, McIntosh JM, J Biol Chem. 1996 Mar 29;271(13):7522-8. PMID:8631783
Page seeded by OCA on Thu Mar 20 12:38:01 2008
Categories: Conus magus | Single protein | Koerber, S C. | Mcintosh, J M. | Olivera, B M. | Rivier, J E. | Shon, K J. | NH2 | Cholinergic modulation | Dopamine release | Neuronal nicotinic acetylcholine receptor inhibitor | Neurotoxin | Presynaptic nicotinic acetylcholine receptor blocker | Subtype specific ligand
