1m4l
From Proteopedia
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| - | [[Image:1m4l.gif|left|200px]] | + | [[Image:1m4l.gif|left|200px]] |
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| - | '''STRUCTURE OF NATIVE CARBOXYPEPTIDASE A AT 1.25 RESOLUTION''' | + | {{Structure |
| + | |PDB= 1m4l |SIZE=350|CAPTION= <scene name='initialview01'>1m4l</scene>, resolution 1.25Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=ZN:ZINC ION'>ZN</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Carboxypeptidase_A Carboxypeptidase A], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.17.1 3.4.17.1] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''STRUCTURE OF NATIVE CARBOXYPEPTIDASE A AT 1.25 RESOLUTION''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1M4L is a [ | + | 1M4L is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M4L OCA]. |
==Reference== | ==Reference== | ||
| - | Refined structure of bovine carboxypeptidase A at 1.25 A resolution., Kilshtain-Vardi A, Glick M, Greenblatt HM, Goldblum A, Shoham G, Acta Crystallogr D Biol Crystallogr. 2003 Feb;59(Pt 2):323-33. Epub 2003, Jan 23. PMID:[http:// | + | Refined structure of bovine carboxypeptidase A at 1.25 A resolution., Kilshtain-Vardi A, Glick M, Greenblatt HM, Goldblum A, Shoham G, Acta Crystallogr D Biol Crystallogr. 2003 Feb;59(Pt 2):323-33. Epub 2003, Jan 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12554943 12554943] |
[[Category: Bos taurus]] | [[Category: Bos taurus]] | ||
[[Category: Carboxypeptidase A]] | [[Category: Carboxypeptidase A]] | ||
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[[Category: Shoham, G.]] | [[Category: Shoham, G.]] | ||
[[Category: ZN]] | [[Category: ZN]] | ||
| - | [[Category: carboxypeptidase | + | [[Category: carboxypeptidase some]] |
[[Category: metalloexoproteinase]] | [[Category: metalloexoproteinase]] | ||
[[Category: metalloproteinase]] | [[Category: metalloproteinase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:38:47 2008'' |
Revision as of 10:38, 20 March 2008
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| , resolution 1.25Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Activity: | Carboxypeptidase A, with EC number 3.4.17.1 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
STRUCTURE OF NATIVE CARBOXYPEPTIDASE A AT 1.25 RESOLUTION
Overview
The crystal structure of the bovine zinc metalloproteinase carboxypeptidase A (CPA) has been refined to 1.25 A resolution based on room-temperature X-ray synchrotron data. The significantly improved structure of CPA at this resolution (anisotropic temperature factors, R factor = 10.4%, R(free) = 14.5%) allowed the modelling of conformational disorders of side chains, improved the description of the protein solvent network (375 water molecules) and provided a more accurate picture of the interactions between the active-site zinc and its ligands. The calculation of standard uncertainties in individual atom positions of the refined model of CPA allowed the deduction of the protonation state of some key residues in the active site and confirmed that Glu72 and Glu270 are negatively charged in the resting state of the enzyme at pH 7.5. These results were further validated by theoretical calculations that showed significant reduction of the pK(a) of these side chains relative to solution values. The distance between the zinc-bound solvent molecule and the metal ion is strongly suggestive of a neutral water molecule and not a hydroxide ion in the resting state of the enzyme. These findings could support both the general acid/general base mechanism, as well as the anhydride mechanism suggested for CPA.
About this Structure
1M4L is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.
Reference
Refined structure of bovine carboxypeptidase A at 1.25 A resolution., Kilshtain-Vardi A, Glick M, Greenblatt HM, Goldblum A, Shoham G, Acta Crystallogr D Biol Crystallogr. 2003 Feb;59(Pt 2):323-33. Epub 2003, Jan 23. PMID:12554943
Page seeded by OCA on Thu Mar 20 12:38:47 2008
