1ma8
From Proteopedia
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| - | [[Image:1ma8.gif|left|200px]] | + | [[Image:1ma8.gif|left|200px]] |
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| - | '''A-DNA decamer GCGTA(UMS)ACGC with incorporated 2'-methylseleno-uridine''' | + | {{Structure |
| + | |PDB= 1ma8 |SIZE=350|CAPTION= <scene name='initialview01'>1ma8</scene>, resolution 1.3Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
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| + | '''A-DNA decamer GCGTA(UMS)ACGC with incorporated 2'-methylseleno-uridine''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1MA8 is a [ | + | 1MA8 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MA8 OCA]. |
==Reference== | ==Reference== | ||
| - | Covalent incorporation of selenium into oligonucleotides for X-ray crystal structure determination via MAD: proof of principle. Multiwavelength anomalous dispersion., Teplova M, Wilds CJ, Wawrzak Z, Tereshko V, Du Q, Carrasco N, Huang Z, Egli M, Biochimie. 2002 Sep;84(9):849-58. PMID:[http:// | + | Covalent incorporation of selenium into oligonucleotides for X-ray crystal structure determination via MAD: proof of principle. Multiwavelength anomalous dispersion., Teplova M, Wilds CJ, Wawrzak Z, Tereshko V, Du Q, Carrasco N, Huang Z, Egli M, Biochimie. 2002 Sep;84(9):849-58. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12458077 12458077] |
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Carrasco, N.]] | [[Category: Carrasco, N.]] | ||
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[[Category: 2'-methylseleno-uridine]] | [[Category: 2'-methylseleno-uridine]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:40:57 2008'' |
Revision as of 10:40, 20 March 2008
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| , resolution 1.3Å | |||||||
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
A-DNA decamer GCGTA(UMS)ACGC with incorporated 2'-methylseleno-uridine
Overview
Selenium was incorporated into an oligodeoxynucleotide in the form of 2'-methylseleno-uridine (U(Se)). The X-ray crystal structure of the duplex left open bracket d(GCGTA)U(Se)d(ACGC) right open bracket (2) was determined by the multiwavelength anomalous dispersion (MAD) technique and refined to a resolution of 1.3 A, demonstrating that selenium can selectively substitute oxygen in DNA and that the resulting compounds are chemically stable. Since derivatization at the 2'-alpha-position with selenium does not affect the preference of the sugar for the C3'-endo conformation, this strategy is suitable for incorporating selenium into RNA. The availability of selenium-containing nucleic acids for crystallographic phasing offers an attractive alternative to the commonly used halogenated pyrimidines.
About this Structure
1MA8 is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Covalent incorporation of selenium into oligonucleotides for X-ray crystal structure determination via MAD: proof of principle. Multiwavelength anomalous dispersion., Teplova M, Wilds CJ, Wawrzak Z, Tereshko V, Du Q, Carrasco N, Huang Z, Egli M, Biochimie. 2002 Sep;84(9):849-58. PMID:12458077
Page seeded by OCA on Thu Mar 20 12:40:57 2008
