1mc3
From Proteopedia
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| - | [[Image:1mc3.jpg|left|200px]] | + | [[Image:1mc3.jpg|left|200px]] |
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| - | '''CRYSTAL STRUCTURE OF RFFH''' | + | {{Structure |
| + | |PDB= 1mc3 |SIZE=350|CAPTION= <scene name='initialview01'>1mc3</scene>, resolution 2.6Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> and <scene name='pdbligand=TTP:THYMIDINE-5'-TRIPHOSPHATE'>TTP</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Glucose-1-phosphate_thymidylyltransferase Glucose-1-phosphate thymidylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.24 2.7.7.24] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''CRYSTAL STRUCTURE OF RFFH''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1MC3 is a [ | + | 1MC3 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MC3 OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of Escherichia coli glucose-1-phosphate thymidylyltransferase (RffH) complexed with dTTP and Mg2+., Sivaraman J, Sauve V, Matte A, Cygler M, J Biol Chem. 2002 Nov 15;277(46):44214-9. Epub 2002 Aug 8. PMID:[http:// | + | Crystal structure of Escherichia coli glucose-1-phosphate thymidylyltransferase (RffH) complexed with dTTP and Mg2+., Sivaraman J, Sauve V, Matte A, Cygler M, J Biol Chem. 2002 Nov 15;277(46):44214-9. Epub 2002 Aug 8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12171937 12171937] |
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Glucose-1-phosphate thymidylyltransferase]] | [[Category: Glucose-1-phosphate thymidylyltransferase]] | ||
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[[Category: montreal-kingston bacterial structural genomics initiative]] | [[Category: montreal-kingston bacterial structural genomics initiative]] | ||
[[Category: rffh]] | [[Category: rffh]] | ||
| - | [[Category: structural | + | [[Category: structural genomic]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:41:41 2008'' |
Revision as of 10:41, 20 March 2008
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| , resolution 2.6Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Activity: | Glucose-1-phosphate thymidylyltransferase, with EC number 2.7.7.24 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF RFFH
Overview
The enzyme glucose-1-phosphate thymidylyltransferase (RffH), the product of the rffh gene, catalyzes one of the steps in the synthesis of enterobacterial common antigen (ECA), a cell surface glycolipid found in Gram-negative enteric bacteria. In Escherichia coli two gene products, RffH and RmlA, catalyze the same enzymatic reaction and are homologous in sequence; however, they are part of different operons and function in different pathways. We report the crystal structure of RffH bound to deoxythymidine triphosphate (dTTP), the phosphate donor, and Mg(2+), refined at 2.6 A to an R-factor of 22.3% (R(free) = 28.4%). The crystal structure of RffH shows a tetrameric enzyme best described as a dimer of dimers. Each monomer has an overall alpha/beta fold and consists of two domains, a larger nucleotide binding domain (residues 1-115, 222-291) and a smaller sugar-binding domain (116-221), with the active site located at the domain interface. The Mg(2+) ion is coordinated by two conserved aspartates and the alpha-phosphate of deoxythymidine triphosphate. Its location corresponds well to that in a structurally similar domain of N-acetylglucosamine-1-phosphate uridylyltransferase (GlmU). Analysis of the RffH, RmlA, and GlmU complexes with substrates and products provides an explanation for their different affinities for Mg(2+) and leads to a proposal for the dynamics along the reaction pathway.
About this Structure
1MC3 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Crystal structure of Escherichia coli glucose-1-phosphate thymidylyltransferase (RffH) complexed with dTTP and Mg2+., Sivaraman J, Sauve V, Matte A, Cygler M, J Biol Chem. 2002 Nov 15;277(46):44214-9. Epub 2002 Aug 8. PMID:12171937
Page seeded by OCA on Thu Mar 20 12:41:41 2008
Categories: Escherichia coli | Glucose-1-phosphate thymidylyltransferase | Single protein | BSGI, Montreal-Kingston Bacterial Structural Genomics Initiative. | Cygler, M. | Matte, A. | Sauve, V. | Sivaraman, J. | MG | TTP | Bsgi | Crystal structure | Glucose-1-phosphate thymidylytransferase | Montreal-kingston bacterial structural genomics initiative | Rffh | Structural genomic
