1meg
From Proteopedia
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| - | [[Image:1meg.jpg|left|200px]] | + | [[Image:1meg.jpg|left|200px]] |
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| - | '''CRYSTAL STRUCTURE OF A CARICAIN D158E MUTANT IN COMPLEX WITH E-64''' | + | {{Structure |
| + | |PDB= 1meg |SIZE=350|CAPTION= <scene name='initialview01'>1meg</scene>, resolution 2.0Å | ||
| + | |SITE= <scene name='pdbsite=ACT:Active+Site'>ACT</scene> | ||
| + | |LIGAND= <scene name='pdbligand=E64:N-[N-[1-HYDROXYCARBOXYETHYL-CARBONYL]LEUCYLAMINO-BUTYL]-GUANIDINE'>E64</scene> and <scene name='pdbligand=EOH:ETHANOL'>EOH</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Caricain Caricain], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.22.30 3.4.22.30] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''CRYSTAL STRUCTURE OF A CARICAIN D158E MUTANT IN COMPLEX WITH E-64''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1MEG is a [ | + | 1MEG is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Carica_papaya Carica papaya]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MEG OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of a caricain D158E mutant in complex with E-64., Katerelos NA, Taylor MA, Scott M, Goodenough PW, Pickersgill RW, FEBS Lett. 1996 Aug 19;392(1):35-9. PMID:[http:// | + | Crystal structure of a caricain D158E mutant in complex with E-64., Katerelos NA, Taylor MA, Scott M, Goodenough PW, Pickersgill RW, FEBS Lett. 1996 Aug 19;392(1):35-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8769310 8769310] |
[[Category: Carica papaya]] | [[Category: Carica papaya]] | ||
[[Category: Caricain]] | [[Category: Caricain]] | ||
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[[Category: thiol protease]] | [[Category: thiol protease]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:42:30 2008'' |
Revision as of 10:42, 20 March 2008
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| , resolution 2.0Å | |||||||
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| Sites: | |||||||
| Ligands: | and | ||||||
| Activity: | Caricain, with EC number 3.4.22.30 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF A CARICAIN D158E MUTANT IN COMPLEX WITH E-64
Overview
The structure of the D158E mutant of caricain (previously known as papaya protease omega) in complex with E-64 has been determined at 2.0 A resolution (overall R factor 19.3%). The structure reveals that the substituted glutamate makes the same pattern of hydrogen bonds as the aspartate in native caricain. This was not anticipated since in the native structure there is insufficient room to accommodate the glutamate side chain. The glutamate is accommodated in the mutant by a local expansion of the structure demonstrating that small structural changes are responsible for the change in activity.
About this Structure
1MEG is a Single protein structure of sequence from Carica papaya. Full crystallographic information is available from OCA.
Reference
Crystal structure of a caricain D158E mutant in complex with E-64., Katerelos NA, Taylor MA, Scott M, Goodenough PW, Pickersgill RW, FEBS Lett. 1996 Aug 19;392(1):35-9. PMID:8769310
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