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3gbs

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[[Image:3gbs.png|left|200px]]
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==Crystal structure of Aspergillus oryzae cutinase==
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<StructureSection load='3gbs' size='340' side='right' caption='[[3gbs]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3gbs]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aspergillus_oryzae Aspergillus oryzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GBS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3GBS FirstGlance]. <br>
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</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">AO090005000029, cutL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5062 Aspergillus oryzae])</td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cutinase Cutinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.74 3.1.1.74] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3gbs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gbs OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3gbs RCSB], [http://www.ebi.ac.uk/pdbsum/3gbs PDBsum]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gb/3gbs_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Cutinases are responsible for hydrolysis of the protective cutin lipid polyester matrix in plants and thus have been exploited for hydrolysis of small molecule esters and polyesters. Here we explore the reactivity, stability, and structure of Aspergillus oryzae cutinase and compare it to the well-studied enzyme from Fusarium solani. Two critical differences are highlighted in the crystallographic analysis of the A. oryzae structure: (i) an additional disulfide bond and (ii) a topologically favored catalytic triad with a continuous and deep groove. These structural features of A. oryzae cutinase are proposed to result in an improved hydrolytic activity and altered substrate specificity profile, enhanced thermostability, and remarkable reactivity toward the degradation of the synthetic polyester polycaprolactone. The results presented here provide insight into engineering new cutinase-inspired biocatalysts with tailor-made properties.
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{{STRUCTURE_3gbs| PDB=3gbs | SCENE= }}
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Structural and functional studies of Aspergillus oryzae cutinase: enhanced thermostability and hydrolytic activity of synthetic ester and polyester degradation.,Liu Z, Gosser Y, Baker PJ, Ravee Y, Lu Z, Alemu G, Li H, Butterfoss GL, Kong XP, Gross R, Montclare JK J Am Chem Soc. 2009 Nov 4;131(43):15711-6. PMID:19810726<ref>PMID:19810726</ref>
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===Crystal structure of Aspergillus oryzae cutinase===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_19810726}}
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==See Also==
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*[[Cutinase|Cutinase]]
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==About this Structure==
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== References ==
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[[3gbs]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aspergillus_oryzae Aspergillus oryzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GBS OCA].
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<references/>
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__TOC__
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==Reference==
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</StructureSection>
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<ref group="xtra">PMID:019810726</ref><references group="xtra"/>
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[[Category: Aspergillus oryzae]]
[[Category: Aspergillus oryzae]]
[[Category: Cutinase]]
[[Category: Cutinase]]
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[[Category: Alemu, G.]]
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[[Category: Alemu, G]]
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[[Category: Gosser, Y.]]
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[[Category: Gosser, Y]]
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[[Category: Kong, X.]]
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[[Category: Kong, X]]
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[[Category: Li, H.]]
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[[Category: Li, H]]
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[[Category: Liu, Z.]]
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[[Category: Liu, Z]]
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[[Category: Lu, Z.]]
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[[Category: Lu, Z]]
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[[Category: Montclare, J.]]
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[[Category: Montclare, J]]
[[Category: Alpha beta hydrolase]]
[[Category: Alpha beta hydrolase]]
[[Category: Hydrolase]]
[[Category: Hydrolase]]
[[Category: Secreted]]
[[Category: Secreted]]
[[Category: Serine esterase]]
[[Category: Serine esterase]]

Revision as of 10:09, 3 December 2014

Crystal structure of Aspergillus oryzae cutinase

3gbs, resolution 1.75Å

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