3g7y

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
m (Protected "3g7y" [edit=sysop:move=sysop])
Line 1: Line 1:
-
[[Image:3g7y.png|left|200px]]
+
==Crystal structure of oxidized Ost6L==
 +
<StructureSection load='3g7y' size='340' side='right' caption='[[3g7y]], [[Resolution|resolution]] 2.21&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[3g7y]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3G7Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3G7Y FirstGlance]. <br>
 +
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">OST6, YML019W ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])</td></tr>
 +
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Dolichyl-diphosphooligosaccharide--protein_glycotransferase Dolichyl-diphosphooligosaccharide--protein glycotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.119 2.4.1.119] </span></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3g7y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3g7y OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3g7y RCSB], [http://www.ebi.ac.uk/pdbsum/3g7y PDBsum]</span></td></tr>
 +
</table>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/g7/3g7y_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Asparagine-linked glycosylation is a common posttranslational modification of diverse secretory and membrane proteins in eukaryotes, where it is catalyzed by the multiprotein complex oligosaccharyltransferase. The functions of the protein subunits of oligoasccharyltransferase, apart from the catalytic Stt3p, are ill defined. Here we describe functional and structural investigations of the Ost3/6p components of the yeast enzyme. Genetic, biochemical and structural analyses of the lumenal domain of Ost6p revealed oxidoreductase activity mediated by a thioredoxin-like fold with a distinctive active-site loop that changed conformation with redox state. We found that mutation of the active-site cysteine residues of Ost6p and its paralogue Ost3p affected the glycosylation efficiency of a subset of glycosylation sites. Our results show that eukaryotic oligosaccharyltransferase is a multifunctional enzyme that acts at the crossroads of protein modification and protein folding.
-
{{STRUCTURE_3g7y| PDB=3g7y | SCENE= }}
+
Oxidoreductase activity of oligosaccharyltransferase subunits Ost3p and Ost6p defines site-specific glycosylation efficiency.,Schulz BL, Stirnimann CU, Grimshaw JP, Brozzo MS, Fritsch F, Mohorko E, Capitani G, Glockshuber R, Grutter MG, Aebi M Proc Natl Acad Sci U S A. 2009 Jul 7;106(27):11061-6. Epub 2009 Jun 23. PMID:19549845<ref>PMID:19549845</ref>
-
===Crystal structure of oxidized Ost6L===
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
 
+
</div>
-
{{ABSTRACT_PUBMED_19549845}}
+
== References ==
-
 
+
<references/>
-
==About this Structure==
+
__TOC__
-
[[3g7y]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3G7Y OCA].
+
</StructureSection>
-
 
+
-
==Reference==
+
-
<ref group="xtra">PMID:019549845</ref><references group="xtra"/>
+
[[Category: Dolichyl-diphosphooligosaccharide--protein glycotransferase]]
[[Category: Dolichyl-diphosphooligosaccharide--protein glycotransferase]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
-
[[Category: Aebi, M.]]
+
[[Category: Aebi, M]]
-
[[Category: Brozzo, M S.]]
+
[[Category: Brozzo, M S]]
-
[[Category: Capitani, G.]]
+
[[Category: Capitani, G]]
-
[[Category: Fritsch, F.]]
+
[[Category: Fritsch, F]]
-
[[Category: Glockshuber, R.]]
+
[[Category: Glockshuber, R]]
-
[[Category: Grimshaw, J P.A.]]
+
[[Category: Grimshaw, J P.A]]
-
[[Category: Gruetter, M G.]]
+
[[Category: Gruetter, M G]]
-
[[Category: Schulz, B L.]]
+
[[Category: Schulz, B L]]
-
[[Category: Stirnimann, C U.]]
+
[[Category: Stirnimann, C U]]
[[Category: Active site loop]]
[[Category: Active site loop]]
[[Category: Membrane]]
[[Category: Membrane]]

Revision as of 10:09, 3 December 2014

Crystal structure of oxidized Ost6L

3g7y, resolution 2.21Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3g7y"
Personal tools