1mf6
From Proteopedia
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| - | [[Image:1mf6.jpg|left|200px]] | + | [[Image:1mf6.jpg|left|200px]] |
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| - | '''Transducin gamma subunit, C-terminal domain 60-71, rhodopsin-bound state: Ensemble of 15 models determined by TrNOE spectroscopy''' | + | {{Structure |
| + | |PDB= 1mf6 |SIZE=350|CAPTION= <scene name='initialview01'>1mf6</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
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| + | '''Transducin gamma subunit, C-terminal domain 60-71, rhodopsin-bound state: Ensemble of 15 models determined by TrNOE spectroscopy''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1MF6 is a [ | + | 1MF6 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MF6 OCA]. |
==Reference== | ==Reference== | ||
| - | Rhodopsin controls a conformational switch on the transducin gamma subunit., Kisselev OG, Downs MA, Structure. 2003 Apr;11(4):367-73. PMID:[http:// | + | Rhodopsin controls a conformational switch on the transducin gamma subunit., Kisselev OG, Downs MA, Structure. 2003 Apr;11(4):367-73. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12679015 12679015] |
[[Category: Bos taurus]] | [[Category: Bos taurus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: transducin]] | [[Category: transducin]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:42:44 2008'' |
Revision as of 10:42, 20 March 2008
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Transducin gamma subunit, C-terminal domain 60-71, rhodopsin-bound state: Ensemble of 15 models determined by TrNOE spectroscopy
Overview
Rhodopsin, a prototypical G protein-coupled receptor, catalyzes the activation of a heterotrimeric G protein, transducin, to initiate a visual signaling cascade in photoreceptor cells. The betagamma subunit complex, especially the C-terminal domain of the transducin gamma subunit, Gtgamma(60-71)farnesyl, plays a pivotal role in allosteric regulation of nucleotide exchange on the transducin alpha subunit by light-activated rhodopsin. We report that this domain is unstructured in the presence of an inactive receptor but forms an amphipathic helix upon rhodopsin activation. A K65E/E66K charge reversal mutant of the gamma subunit has diminished interactions with the receptor and fails to adopt the helical conformation. The identification of this conformational switch provides a mechanism for active GPCR utilization of the betagamma complex in signal transfer to G proteins.
About this Structure
1MF6 is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.
Reference
Rhodopsin controls a conformational switch on the transducin gamma subunit., Kisselev OG, Downs MA, Structure. 2003 Apr;11(4):367-73. PMID:12679015
Page seeded by OCA on Thu Mar 20 12:42:44 2008
