1mfq
From Proteopedia
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| - | [[Image:1mfq.gif|left|200px]] | + | [[Image:1mfq.gif|left|200px]] |
| - | + | ||
| - | '''Crystal Structure Analysis of a Ternary S-Domain Complex of Human Signal Recognition Particle''' | + | {{Structure |
| + | |PDB= 1mfq |SIZE=350|CAPTION= <scene name='initialview01'>1mfq</scene>, resolution 3.10Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> and <scene name='pdbligand=CL:CHLORIDE ION'>CL</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= SRP19 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), SRP54 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal Structure Analysis of a Ternary S-Domain Complex of Human Signal Recognition Particle''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1MFQ is a [ | + | 1MFQ is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MFQ OCA]. |
==Reference== | ==Reference== | ||
| - | Induced structural changes of 7SL RNA during the assembly of human signal recognition particle., Kuglstatter A, Oubridge C, Nagai K, Nat Struct Biol. 2002 Oct;9(10):740-4. PMID:[http:// | + | Induced structural changes of 7SL RNA during the assembly of human signal recognition particle., Kuglstatter A, Oubridge C, Nagai K, Nat Struct Biol. 2002 Oct;9(10):740-4. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12244299 12244299] |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: rna-protein complex]] | [[Category: rna-protein complex]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:43:00 2008'' |
Revision as of 10:43, 20 March 2008
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| , resolution 3.10Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Gene: | SRP19 (Homo sapiens), SRP54 (Homo sapiens) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure Analysis of a Ternary S-Domain Complex of Human Signal Recognition Particle
Overview
The eukaryotic signal recognition particle (SRP) is a cytoplasmic ribonucleoprotein particle that targets secretory and membrane proteins to the endoplasmic reticulum. The binding of SRP54 to the S domain of 7SL RNA is highly dependent on SRP19. Here we present the crystal structure of a human SRP ternary complex consisting of SRP19, the M domain of SRP54 and the S domain of 7SL RNA. Upon binding of the M domain of SRP54 to the 7SL RNA-SRP19 complex, the asymmetric loop of helix 8 in 7SL RNA collapses. The bases of the four nucleotides in the long strand of the asymmetric loop continuously stack and interact with the M domain, whereas the two adenines in the short strand flip out and form two A-minor motifs with helix 6. This stabilizing interaction is only possible when helix 6 has been positioned parallel to helix 8 by the prior binding of SRP19 to the tetraloops of helices 6 and 8. Hence, the crystal structure of the ternary complex suggests why SRP19 is necessary for the stable binding of SRP54 to the S domain RNA.
About this Structure
1MFQ is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Induced structural changes of 7SL RNA during the assembly of human signal recognition particle., Kuglstatter A, Oubridge C, Nagai K, Nat Struct Biol. 2002 Oct;9(10):740-4. PMID:12244299
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