3gcd

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[[Image:3gcd.png|left|200px]]
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==Structure of the V. cholerae RTX cysteine protease domain in complex with an aza-Leucine peptide inhibitor==
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<StructureSection load='3gcd' size='340' side='right' caption='[[3gcd]], [[Resolution|resolution]] 2.35&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3gcd]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GCD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3GCD FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AZ0:ETHYL+(5S,8S,14S)-14-HYDROXY-5,8,11-TRIS(2-METHYLPROPYL)-3,6,9,12-TETRAOXO-1-PHENYL-2-OXA-4,7,10,11-TETRAAZAPENTADECAN-15-OATE'>AZ0</scene>, <scene name='pdbligand=IHP:INOSITOL+HEXAKISPHOSPHATE'>IHP</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">rtxA, vc1451, VC_1451 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=666 Vibrio cholerae])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3gcd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gcd OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3gcd RCSB], [http://www.ebi.ac.uk/pdbsum/3gcd PDBsum]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gc/3gcd_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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MARTX toxins modulate the virulence of a number of Gram-negative Vibrio species. This family of toxins is defined by the presence of a cysteine protease domain (CPD), which proteolytically activates the Vibrio cholerae MARTX toxin. Although recent structural studies of the CPD have uncovered a new allosteric activation mechanism, the mechanism of CPD substrate recognition or toxin processing is unknown. Here we show that interdomain cleavage of MARTXVc enhances effector domain function. We also identify the first small-molecule inhibitors of this protease domain and present the 2.35-A structure of the CPD bound to one of these inhibitors. This structure, coupled with biochemical and mutational studies of the toxin, reveals the molecular basis of CPD substrate specificity and underscores the evolutionary relationship between the CPD and the clan CD caspase proteases. These studies are likely to prove valuable for devising new antitoxin strategies for a number of bacterial pathogens.
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{{STRUCTURE_3gcd| PDB=3gcd | SCENE= }}
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Mechanistic and structural insights into the proteolytic activation of Vibrio cholerae MARTX toxin.,Shen A, Lupardus PJ, Albrow VE, Guzzetta A, Powers JC, Garcia KC, Bogyo M Nat Chem Biol. 2009 Jul;5(7):469-78. PMID:19465933<ref>PMID:19465933</ref>
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===Structure of the V. cholerae RTX cysteine protease domain in complex with an aza-Leucine peptide inhibitor===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_19465933}}
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== References ==
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<references/>
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==About this Structure==
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__TOC__
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[[3gcd]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GCD OCA].
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</StructureSection>
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==Reference==
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<ref group="xtra">PMID:019465933</ref><references group="xtra"/>
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[[Category: Vibrio cholerae]]
[[Category: Vibrio cholerae]]
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[[Category: Bogyo, M.]]
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[[Category: Bogyo, M]]
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[[Category: Garcia, K C.]]
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[[Category: Garcia, K C]]
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[[Category: Lupardus, P J.]]
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[[Category: Lupardus, P J]]
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[[Category: Shen, A.]]
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[[Category: Shen, A]]
[[Category: Aza-leu]]
[[Category: Aza-leu]]
[[Category: Aza-peptide]]
[[Category: Aza-peptide]]

Revision as of 10:15, 3 December 2014

Structure of the V. cholerae RTX cysteine protease domain in complex with an aza-Leucine peptide inhibitor

3gcd, resolution 2.35Å

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