2yb6
From Proteopedia
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| - | [[ | + | ==NATIVE HUMAN RAD6== |
| + | <StructureSection load='2yb6' size='340' side='right' caption='[[2yb6]], [[Resolution|resolution]] 1.50Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[2yb6]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YB6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2YB6 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2ybf|2ybf]], [[2y4w|2y4w]], [[1nxa|1nxa]], [[1jas|1jas]]</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2yb6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2yb6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2yb6 RCSB], [http://www.ebi.ac.uk/pdbsum/2yb6 PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | In ubiquitin conjugation, different combinations of E2 and E3 enzymes catalyse either monoubiquitination or ubiquitin chain formation. The E2/E3 complex Rad6/Rad18 exclusively monoubiquitinates the proliferating cell nuclear antigen (PCNA) to signal for "error prone" DNA damage tolerance, whereas a different set of conjugation enzymes is required for ubiquitin chain formation on PCNA. Here we show that human E2 enzyme Rad6b is intrinsically capable of catalyzing ubiquitin chain formation. This activity is prevented during PCNA ubiquitination by the interaction of Rad6 with E3 enzyme Rad18. Using NMR and X-ray crystallography we show that the R6BD of Rad18 inhibits this activity by competing with ubiquitin for a noncovalent "backside" binding site on Rad6. Our findings provide mechanistic insights into how E3 enzymes can regulate the ubiquitin conjugation process. | ||
| - | + | E3 ligase Rad18 promotes monoubiquitination rather than ubiquitin chain formation by E2 enzyme Rad6.,Hibbert RG, Huang A, Boelens R, Sixma TK Proc Natl Acad Sci U S A. 2011 Apr 5;108(14):5590-5. Epub 2011 Mar 21. PMID:21422291<ref>PMID:21422291</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| + | </div> | ||
| - | + | ==See Also== | |
| - | + | *[[Ubiquitin conjugating enzyme|Ubiquitin conjugating enzyme]] | |
| - | == | + | == References == |
| - | [[ | + | <references/> |
| - | + | __TOC__ | |
| - | == | + | </StructureSection> |
| - | < | + | |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
| - | [[Category: Hibbert, R G | + | [[Category: Hibbert, R G]] |
| - | [[Category: Sixma, T K | + | [[Category: Sixma, T K]] |
[[Category: Ligase]] | [[Category: Ligase]] | ||
[[Category: Translesion synthesis]] | [[Category: Translesion synthesis]] | ||
[[Category: Ubiquitin chain synthesis]] | [[Category: Ubiquitin chain synthesis]] | ||
Revision as of 10:26, 3 December 2014
NATIVE HUMAN RAD6
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