1mi4
From Proteopedia
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| - | [[Image:1mi4.gif|left|200px]] | + | [[Image:1mi4.gif|left|200px]] |
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| - | '''Glyphosate insensitive G96A mutant EPSP synthase liganded with shikimate-3-phosphate''' | + | {{Structure |
| + | |PDB= 1mi4 |SIZE=350|CAPTION= <scene name='initialview01'>1mi4</scene>, resolution 1.7Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=S3P:SHIKIMATE-3-PHOSPHATE'>S3P</scene> and <scene name='pdbligand=FMT:FORMIC ACID'>FMT</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/3-phosphoshikimate_1-carboxyvinyltransferase 3-phosphoshikimate 1-carboxyvinyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.19 2.5.1.19] | ||
| + | |GENE= aroA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | ||
| + | }} | ||
| + | |||
| + | '''Glyphosate insensitive G96A mutant EPSP synthase liganded with shikimate-3-phosphate''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1MI4 is a [ | + | 1MI4 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MI4 OCA]. |
==Reference== | ==Reference== | ||
| - | How the mutation glycine96 to alanine confers glyphosate insensitivity to 5-enolpyruvyl shikimate-3-phosphate synthase from Escherichia coli., Eschenburg S, Healy ML, Priestman MA, Lushington GH, Schonbrunn E, Planta. 2002 Nov;216(1):129-35. Epub 2002 Nov 12. PMID:[http:// | + | How the mutation glycine96 to alanine confers glyphosate insensitivity to 5-enolpyruvyl shikimate-3-phosphate synthase from Escherichia coli., Eschenburg S, Healy ML, Priestman MA, Lushington GH, Schonbrunn E, Planta. 2002 Nov;216(1):129-35. Epub 2002 Nov 12. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12430021 12430021] |
[[Category: 3-phosphoshikimate 1-carboxyvinyltransferase]] | [[Category: 3-phosphoshikimate 1-carboxyvinyltransferase]] | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
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[[Category: inside-out alpha-beta barrel]] | [[Category: inside-out alpha-beta barrel]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:43:41 2008'' |
Revision as of 10:43, 20 March 2008
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| , resolution 1.7Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Gene: | aroA (Escherichia coli) | ||||||
| Activity: | 3-phosphoshikimate 1-carboxyvinyltransferase, with EC number 2.5.1.19 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Glyphosate insensitive G96A mutant EPSP synthase liganded with shikimate-3-phosphate
Overview
The enzyme 5-enolpyruvyl shikimate-3-phosphate (EPSP) synthase (EC 2.5.1.19) is essential for the biosynthesis of aromatic compounds in plants and microbes and is the unique target of the herbicide glyphosate. One of the first glyphosate-insensitive enzymes reported was a Gly96Ala mutant of EPSP synthase from Klebsiella pneumoniae. We have introduced this single-site mutation into the highly homologous EPSP synthase from Escherichia coli. The mutant enzyme is insensitive to glyphosate with unaltered affinity for its first substrate, shikimate-3-phosphate (S3P), but displays a 30-fold lower affinity for its second substrate, phosphoenolpyruvate (PEP). Using X-ray crystallography, we solved the structure of Gly96Ala-EPSP synthase liganded with S3P to 0.17 nm resolution. The crystal structure shows that the additional methyl group from Ala96 protrudes into the active site of the enzyme. While the interactions between enzyme and S3P remain unaffected, the accessible volume for glyphosate binding is substantially reduced. Exploiting the crystallographic results for molecular modeling, we demonstrate that PEP but not glyphosate can be docked in the Gly96Ala-modified binding site. The predicted PEP binding site satisfies the earlier proposed interaction pattern for PEP with EPSP synthase and corroborates the assumption that glyphosate and PEP target the same binding site.
About this Structure
1MI4 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
How the mutation glycine96 to alanine confers glyphosate insensitivity to 5-enolpyruvyl shikimate-3-phosphate synthase from Escherichia coli., Eschenburg S, Healy ML, Priestman MA, Lushington GH, Schonbrunn E, Planta. 2002 Nov;216(1):129-35. Epub 2002 Nov 12. PMID:12430021
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