3foz

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[[Image:3foz.png|left|200px]]
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==Structure of E. coli Isopentenyl-tRNA transferase in complex with E. coli tRNA(Phe)==
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<StructureSection load='3foz' size='340' side='right' caption='[[3foz]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3foz]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k-12 Escherichia coli k-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FOZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3FOZ FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">b4171, E. coli, JW4129, miaA, trpX ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 Escherichia coli K-12])</td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/tRNA_dimethylallyltransferase tRNA dimethylallyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.75 2.5.1.75] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3foz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3foz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3foz RCSB], [http://www.ebi.ac.uk/pdbsum/3foz PDBsum]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fo/3foz_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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tRNAs that read codons starting with U are usually modified at their A37 by isopentenyl-tRNA transferases in order to minimize peptidyl-tRNA slippage in translation. The consensus substrate requirements of E. coli's isopentenyl-tRNA transferase, MiaA, have been the focus of extensive study. However, the molecular basis of tRNA-MiaA recognition remains unknown. Here, we describe the 2.5 A crystal structure of MiaA in complex with substrate tRNA(Phe). Comparative structural analysis reveals that the enzymatic reaction involves an RNA-protein mutually induced fit mechanism in which large domain movements in MiaA provoke the partial unfolding of the substrate tRNA anticodon loop. In addition, we show how substrate tRNAs are recognized by MiaA through a combination of direct and indirect sequence readouts.
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{{STRUCTURE_3foz| PDB=3foz | SCENE= }}
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RNA-protein mutually induced Fit: Structure of E. coli isopentenyl-tRNA transferase in complex with tRNA(Phe).,Seif E, Hallberg BM J Biol Chem. 2009 Jan 21. PMID:19158097<ref>PMID:19158097</ref>
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===Structure of E. coli Isopentenyl-tRNA transferase in complex with E. coli tRNA(Phe)===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_19158097}}
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== References ==
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<references/>
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==About this Structure==
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__TOC__
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[[3foz]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k-12 Escherichia coli k-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FOZ OCA].
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</StructureSection>
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==Reference==
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<ref group="xtra">PMID:019158097</ref><references group="xtra"/>
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[[Category: Escherichia coli k-12]]
[[Category: Escherichia coli k-12]]
[[Category: TRNA dimethylallyltransferase]]
[[Category: TRNA dimethylallyltransferase]]
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[[Category: Hallberg, B M.]]
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[[Category: Hallberg, B M]]
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[[Category: Seif, E.]]
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[[Category: Seif, E]]
[[Category: Isopentenyl-trna transferase]]
[[Category: Isopentenyl-trna transferase]]
[[Category: Miaa]]
[[Category: Miaa]]

Revision as of 10:28, 3 December 2014

Structure of E. coli Isopentenyl-tRNA transferase in complex with E. coli tRNA(Phe)

3foz, resolution 2.50Å

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