Poly(A) Polymerase

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[[Image:2q66.png|left|170px]]
[[Image:2q66.png|left|170px]]
[[Poly(A) Polymerase]] (polynucleotide adenylytransferase, [[EC Number|EC]] 2.7.7.19) is the enzyme responsible for adding a polyadenine tail to the 3' end of a nascent pre-mRNA transcript. Its substrates are ATP and RNA. The poly(A) tail that poly-A polymerase adds to the 3' end of the pre-mRNA transcript is important for nuclear export, translation and stability of the mRNA.
[[Poly(A) Polymerase]] (polynucleotide adenylytransferase, [[EC Number|EC]] 2.7.7.19) is the enzyme responsible for adding a polyadenine tail to the 3' end of a nascent pre-mRNA transcript. Its substrates are ATP and RNA. The poly(A) tail that poly-A polymerase adds to the 3' end of the pre-mRNA transcript is important for nuclear export, translation and stability of the mRNA.
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__NOTOC__
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==Determinants of ATP Recognition==
==Determinants of ATP Recognition==
<StructureSection load='2q66' scene='Proteopedia:Main_page_develop/2q66_initial/3' size='500' side='right' caption='Structure of Yeast Poly(A) Polymerase with ATP, Mg+2 ion (green) and oligo(A) (PDB entry [[2q66]])'>
<StructureSection load='2q66' scene='Proteopedia:Main_page_develop/2q66_initial/3' size='500' side='right' caption='Structure of Yeast Poly(A) Polymerase with ATP, Mg+2 ion (green) and oligo(A) (PDB entry [[2q66]])'>
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Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
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{{#tree:id=OrganizedByTopic|openlevels=0|
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[[3aqk]], [[3aql]], [[3aqm]], [[3aqn]] – PAP residues 18-431 – ''Escherichia coli''<BR />
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*Poly(A) polymerase
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[[2hhp]], [[2o1p]] – yPAP - yeast<BR />
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[[1q79]], [[1f5a]] - bPAP-α - bovine<br />
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[[4lt6]] – hPAP-Υ – human<br />
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===Poly(A) polymerase complex===
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**[[3aqk]], [[3aql]], [[3aqm]], [[3aqn]] – PAP residues 18-431 – ''Escherichia coli''<BR />
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**[[2hhp]], [[2o1p]] – yPAP - yeast<BR />
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**[[1q79]], [[1f5a]] - bPAP-α - bovine<br />
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**[[4lt6]] – hPAP-Υ – human<br />
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[[3er8]], [[3er9]], [[3erc]] – VvPAP VP55 + RNA - Vaccinia virus<BR />
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*Poly(A) polymerase complex
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[[2ga9]] - VvPAP VP55 + VP39 + ATP<BR />
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[[2gaf]] - VvPAP VP55 (mutant) + VP39<BR />
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[[1jtf]], [[2vp3]] - VvPAP VP39 residues 1-307 (mutant) + M7GPPPG + SAH<BR />
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[[1b42]], [[1bky]], [[1eqa]], [[3mag]], [[3mct]], [[4dcg]], [[1av6]], [[1p39]], [[1v39]]. [[1jsz]] - VvPAP VP39 residues 1-297 (mutant) + purine derivative + SAH<BR />
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[[1vp3]] - VvPAP VP39 + SAH<BR />
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[[1vp9]], [[1eam]], [[1jte]] - VvPAP VP39 (mutant) + SAH<BR />
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[[1vpt]] - VvPAP VP39 (mutant) + SAM<BR />
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[[3c66]] - yPAP residues 1-526 + Fip1 peptide <BR />
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[[2q66]] – yPAP + polyA + ATP<BR />
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[[1fa0]] - yPAP + Mn + ATP<br />
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[[1q78]] – bPAP-α + Mg + ATP<BR />
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**[[3er8]], [[3er9]], [[3erc]] – VvPAP VP55 + RNA - Vaccinia virus<BR />
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**[[2ga9]] - VvPAP VP55 + VP39 + ATP<BR />
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**[[2gaf]] - VvPAP VP55 (mutant) + VP39<BR />
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**[[1jtf]], [[2vp3]] - VvPAP VP39 residues 1-307 (mutant) + M7GPPPG + SAH<BR />
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**[[1b42]], [[1bky]], [[1eqa]], [[3mag]], [[3mct]], [[4dcg]], [[1av6]], [[1p39]], [[1v39]]. [[1jsz]] - VvPAP VP39 residues 1-297 (mutant) + purine derivative + SAH<BR />
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**[[1vp3]] - VvPAP VP39 + SAH<BR />
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**[[1vp9]], [[1eam]], [[1jte]] - VvPAP VP39 (mutant) + SAH<BR />
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**[[1vpt]] - VvPAP VP39 (mutant) + SAM<BR />
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**[[3c66]] - yPAP residues 1-526 + Fip1 peptide <BR />
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**[[2q66]] – yPAP + polyA + ATP<BR />
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**[[1fa0]] - yPAP + Mn + ATP<br />
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**[[1q78]] – bPAP-α + Mg + ATP<BR />
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}}

Revision as of 12:01, 3 December 2014

Poly(A) Polymerase (polynucleotide adenylytransferase, EC 2.7.7.19) is the enzyme responsible for adding a polyadenine tail to the 3' end of a nascent pre-mRNA transcript. Its substrates are ATP and RNA. The poly(A) tail that poly-A polymerase adds to the 3' end of the pre-mRNA transcript is important for nuclear export, translation and stability of the mRNA.

Contents

Determinants of ATP Recognition

Structure of Yeast Poly(A) Polymerase with ATP, Mg+2 ion (green) and oligo(A) (PDB entry 2q66)

Drag the structure with the mouse to rotate
This section complements the article on Poly(A) Polymerase in the Molecule of the Month Series. See also Teaching Scenes, Tutorials, and Educators' Pages.

3D structures of poly(A) polymerase

Updated on 03-December-2014


Additional Resources

For additional information, see: Translation
For additional examples of transferases, see: Transferase

Reference

Mechanism of poly(A) polymerase: structure of the enzyme-MgATP-RNA ternary complex and kinetic analysis., Balbo PB, Bohm A, Structure. 2007 Sep;15(9):1117-31. PMID:17850751

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