1mj1
From Proteopedia
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| - | [[Image:1mj1.gif|left|200px]] | + | [[Image:1mj1.gif|left|200px]] |
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| - | '''FITTING THE TERNARY COMPLEX OF EF-Tu/tRNA/GTP AND RIBOSOMAL PROTEINS INTO A 13 A CRYO-EM MAP OF THE COLI 70S RIBOSOME''' | + | {{Structure |
| + | |PDB= 1mj1 |SIZE=350|CAPTION= <scene name='initialview01'>1mj1</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
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| + | '''FITTING THE TERNARY COMPLEX OF EF-Tu/tRNA/GTP AND RIBOSOMAL PROTEINS INTO A 13 A CRYO-EM MAP OF THE COLI 70S RIBOSOME''' | ||
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==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1MJ1 is a [ | + | 1MJ1 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MJ1 OCA]. |
==Reference== | ==Reference== | ||
| - | Ribosome interactions of aminoacyl-tRNA and elongation factor Tu in the codon-recognition complex., Stark H, Rodnina MV, Wieden HJ, Zemlin F, Wintermeyer W, van Heel M, Nat Struct Biol. 2002 Nov;9(11):849-54. PMID:[http:// | + | Ribosome interactions of aminoacyl-tRNA and elongation factor Tu in the codon-recognition complex., Stark H, Rodnina MV, Wieden HJ, Zemlin F, Wintermeyer W, van Heel M, Nat Struct Biol. 2002 Nov;9(11):849-54. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12379845 12379845] |
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: low resolution model ternary complex]] | [[Category: low resolution model ternary complex]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:43:58 2008'' |
Revision as of 10:44, 20 March 2008
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FITTING THE TERNARY COMPLEX OF EF-Tu/tRNA/GTP AND RIBOSOMAL PROTEINS INTO A 13 A CRYO-EM MAP OF THE COLI 70S RIBOSOME
Overview
The mRNA codon in the ribosomal A-site is recognized by aminoacyl-tRNA (aa-tRNA) in a ternary complex with elongation factor Tu (EF-Tu) and GTP. Here we report the 13 A resolution three-dimensional reconstruction determined by cryo-electron microscopy of the kirromycin-stalled codon-recognition complex. The structure of the ternary complex is distorted by binding of the tRNA anticodon arm in the decoding center. The aa-tRNA interacts with 16S rRNA, helix 69 of 23S rRNA and proteins S12 and L11, while the sarcin-ricin loop of 23S rRNA contacts domain 1 of EF-Tu near the nucleotide-binding pocket. These results provide a detailed snapshot view of an important functional state of the ribosome and suggest mechanisms of decoding and GTPase activation.
About this Structure
1MJ1 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Ribosome interactions of aminoacyl-tRNA and elongation factor Tu in the codon-recognition complex., Stark H, Rodnina MV, Wieden HJ, Zemlin F, Wintermeyer W, van Heel M, Nat Struct Biol. 2002 Nov;9(11):849-54. PMID:12379845
Page seeded by OCA on Thu Mar 20 12:43:58 2008
