3izv

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:3izv.png|left|200px]]
+
==Structural insights into cognate vs. near-cognate discrimination during decoding. This entry contains the small subunit of a ribosome programmed with a near-cognate codon, A/T-site tRNA, P-site tRNA, mRNA and EF-Tu==
 +
<StructureSection load='3izv' size='340' side='right' caption='[[3izv]], [[Resolution|resolution]] 13.20&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[3izv]] is a 25 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli], [http://en.wikipedia.org/wiki/Escherichia_coli_dh1 Escherichia coli dh1], [http://en.wikipedia.org/wiki/Escherichia_coli_k-12 Escherichia coli k-12] and [http://en.wikipedia.org/wiki/Escherichia_coli_o157:h7 Escherichia coli o157:h7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IZV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3IZV FirstGlance]. <br>
 +
</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=2MG:2N-METHYLGUANOSINE-5-MONOPHOSPHATE'>2MG</scene>, <scene name='pdbligand=3AU:3-[(3S)-3-AMINO-3-CARBOXYPROPYL]URIDINE+5-(DIHYDROGEN+PHOSPHATE)'>3AU</scene>, <scene name='pdbligand=4OC:4N,O2-METHYLCYTIDINE-5-MONOPHOSPHATE'>4OC</scene>, <scene name='pdbligand=4SU:4-THIOURIDINE-5-MONOPHOSPHATE'>4SU</scene>, <scene name='pdbligand=5MC:5-METHYLCYTIDINE-5-MONOPHOSPHATE'>5MC</scene>, <scene name='pdbligand=5MU:5-METHYLURIDINE+5-MONOPHOSPHATE'>5MU</scene>, <scene name='pdbligand=7MG:7N-METHYL-8-HYDROGUANOSINE-5-MONOPHOSPHATE'>7MG</scene>, <scene name='pdbligand=H2U:5,6-DIHYDROURIDINE-5-MONOPHOSPHATE'>H2U</scene>, <scene name='pdbligand=MA6:6N-DIMETHYLADENOSINE-5-MONOPHOSHATE'>MA6</scene>, <scene name='pdbligand=MIA:2-METHYLTHIO-N6-ISOPENTENYL-ADENOSINE-5-MONOPHOSPHATE'>MIA</scene>, <scene name='pdbligand=PSU:PSEUDOURIDINE-5-MONOPHOSPHATE'>PSU</scene>, <scene name='pdbligand=UR3:3-METHYLURIDINE-5-MONOPHOSHATE'>UR3</scene></td></tr>
 +
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3izt|3izt]]</td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3izv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3izv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3izv RCSB], [http://www.ebi.ac.uk/pdbsum/3izv PDBsum]</span></td></tr>
 +
</table>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
The structural basis of the tRNA selection process is investigated by cryo-electron microscopy of ribosomes programmed with UGA codons and incubated with ternary complex (TC) containing the near-cognate Trp-tRNA(Trp) in the presence of kirromycin. Going through more than 350 000 images and employing image classification procedures, we find approximately 8% in which the TC is bound to the ribosome. The reconstructed 3D map provides a means to characterize the arrangement of the near-cognate aa-tRNA with respect to elongation factor Tu (EF-Tu) and the ribosome, as well as the domain movements of the ribosome. One of the interesting findings is that near-cognate tRNA's acceptor stem region is flexible and CCA end becomes disordered. The data bring direct structural insights into the induced-fit mechanism of decoding by the ribosome, as the analysis of the interactions between small and large ribosomal subunit, aa-tRNA and EF-Tu and comparison with the cognate case (UGG codon) offers clues on how the conformational signals conveyed to the GTPase differ in the two cases.
-
{{STRUCTURE_3izv| PDB=3izv | SCENE= }}
+
Structural insights into cognate versus near-cognate discrimination during decoding.,Agirrezabala X, Schreiner E, Trabuco LG, Lei J, Ortiz-Meoz RF, Schulten K, Green R, Frank J EMBO J. 2011 Mar 4. PMID:21378755<ref>PMID:21378755</ref>
-
===Structural insights into cognate vs. near-cognate discrimination during decoding. This entry contains the small subunit of a ribosome programmed with a near-cognate codon, A/T-site tRNA, P-site tRNA, mRNA and EF-Tu===
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
 
+
</div>
-
{{ABSTRACT_PUBMED_21378755}}
+
-
 
+
-
==About this Structure==
+
-
[[3izv]] is a 25 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli], [http://en.wikipedia.org/wiki/Escherichia_coli_dh1 Escherichia coli dh1], [http://en.wikipedia.org/wiki/Escherichia_coli_k-12 Escherichia coli k-12] and [http://en.wikipedia.org/wiki/Escherichia_coli_o157:h7 Escherichia coli o157:h7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IZV OCA].
+
==See Also==
==See Also==
*[[Elongation factor|Elongation factor]]
*[[Elongation factor|Elongation factor]]
-
*[[Ribosomal protein S10|Ribosomal protein S10]]
+
*[[Ribosome 3D structures|Ribosome 3D structures]]
-
*[[Ribosomal protein S11|Ribosomal protein S11]]
+
== References ==
-
*[[Ribosomal protein S12|Ribosomal protein S12]]
+
<references/>
-
*[[Ribosomal protein S13|Ribosomal protein S13]]
+
__TOC__
-
*[[Ribosomal protein S14|Ribosomal protein S14]]
+
</StructureSection>
-
*[[Ribosomal protein S15|Ribosomal protein S15]]
+
-
*[[Ribosomal protein S16|Ribosomal protein S16]]
+
-
*[[Ribosomal protein S17|Ribosomal protein S17]]
+
-
*[[Ribosomal protein S18|Ribosomal protein S18]]
+
-
*[[Ribosomal protein S19|Ribosomal protein S19]]
+
-
*[[Ribosomal protein S2|Ribosomal protein S2]]
+
-
*[[Ribosomal protein S20|Ribosomal protein S20]]
+
-
*[[Ribosomal protein S21|Ribosomal protein S21]]
+
-
*[[Ribosomal protein S3|Ribosomal protein S3]]
+
-
*[[Ribosomal protein S4|Ribosomal protein S4]]
+
-
*[[Ribosomal protein S5|Ribosomal protein S5]]
+
-
*[[Ribosomal protein S6|Ribosomal protein S6]]
+
-
*[[Ribosomal protein S7|Ribosomal protein S7]]
+
-
*[[Ribosomal protein S8|Ribosomal protein S8]]
+
-
*[[Ribosomal protein S9|Ribosomal protein S9]]
+
-
 
+
-
==Reference==
+
-
<ref group="xtra">PMID:021378755</ref><references group="xtra"/>
+
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Escherichia coli dh1]]
[[Category: Escherichia coli dh1]]
[[Category: Escherichia coli k-12]]
[[Category: Escherichia coli k-12]]
[[Category: Escherichia coli o157:h7]]
[[Category: Escherichia coli o157:h7]]
-
[[Category: Agirrezabala, X.]]
+
[[Category: Agirrezabala, X]]
-
[[Category: Frank, J.]]
+
[[Category: Frank, J]]
-
[[Category: Green, R.]]
+
[[Category: Green, R]]
-
[[Category: Lei, J.]]
+
[[Category: Lei, J]]
-
[[Category: Ortiz-Meoz, R F.]]
+
[[Category: Ortiz-Meoz, R F]]
-
[[Category: Schreiner, E.]]
+
[[Category: Schreiner, E]]
-
[[Category: Schulten, K.]]
+
[[Category: Schulten, K]]
-
[[Category: Trabuco, L G.]]
+
[[Category: Trabuco, L G]]
[[Category: Cryoem]]
[[Category: Cryoem]]
[[Category: Near-cognate]]
[[Category: Near-cognate]]

Revision as of 08:41, 8 December 2014

Structural insights into cognate vs. near-cognate discrimination during decoding. This entry contains the small subunit of a ribosome programmed with a near-cognate codon, A/T-site tRNA, P-site tRNA, mRNA and EF-Tu

3izv, resolution 13.20Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3izv"
Personal tools