3j1n
From Proteopedia
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| - | [[ | + | ==Cryo-EM map of a yeast minimal preinitiation complex interacting with the Mediator Head module== |
| + | <StructureSection load='3j1n' size='340' side='right' caption='[[3j1n]], [[Resolution|resolution]] 16.00Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[3j1n]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J1N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3J1N FirstGlance]. <br> | ||
| + | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3j1o|3j1o]]</td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j1n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j1n OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3j1n RCSB], [http://www.ebi.ac.uk/pdbsum/3j1n PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Mediator, a large (21 polypeptides, MW approximately 1 MDa) complex conserved throughout eukaryotes, plays an essential role in control of gene expression by conveying regulatory signals that influence the activity of the preinitiation complex. However, the precise mode of interaction between Mediator and RNA polymerase II (RNAPII), and the mechanism of regulation by Mediator remain elusive. We used cryo-electron microscopy and reconstituted in vitro transcription assays to characterize a transcriptionally-active complex including the Mediator Head module and components of a minimum preinitiation complex (RNAPII, TFIIF, TFIIB, TBP, and promoter DNA). Our results reveal how the Head interacts with RNAPII, affecting its conformation and function. | ||
| - | + | Interaction of the mediator head module with RNA polymerase II.,Cai G, Chaban YL, Imasaki T, Kovacs JA, Calero G, Penczek PA, Takagi Y, Asturias FJ Structure. 2012 May 9;20(5):899-910. doi: 10.1016/j.str.2012.02.023. PMID:22579255<ref>PMID:22579255</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| + | </div> | ||
| - | + | ==See Also== | |
| - | + | *[[RNA polymerase|RNA polymerase]] | |
| - | == | + | == References == |
| - | [[ | + | <references/> |
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: DNA-directed RNA polymerase]] | [[Category: DNA-directed RNA polymerase]] | ||
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
| - | [[Category: Asturias, F J | + | [[Category: Asturias, F J]] |
| - | [[Category: Imasaki, T | + | [[Category: Imasaki, T]] |
[[Category: Mediator head module]] | [[Category: Mediator head module]] | ||
[[Category: Preinitiation complex]] | [[Category: Preinitiation complex]] | ||
[[Category: Transcription]] | [[Category: Transcription]] | ||
[[Category: Transferase]] | [[Category: Transferase]] | ||
Revision as of 08:42, 8 December 2014
Cryo-EM map of a yeast minimal preinitiation complex interacting with the Mediator Head module
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