3hp4

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[[Image:3hp4.png|left|200px]]
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==Crystal structure of psychrotrophic esterase EstA from Pseudoalteromonas sp. 643A inhibited by monoethylphosphonate==
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<StructureSection load='3hp4' size='340' side='right' caption='[[3hp4]], [[Resolution|resolution]] 1.35&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3hp4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudoalteromonas_sp. Pseudoalteromonas sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HP4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3HP4 FirstGlance]. <br>
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</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MIR:MONOETHYLPHOSPHORYLSERINE'>MIR</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">estA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=53249 Pseudoalteromonas sp.])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hp4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hp4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3hp4 RCSB], [http://www.ebi.ac.uk/pdbsum/3hp4 PDBsum]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hp/3hp4_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The crystal structure of the esterase EstA from the cold-adapted bacterium Pseudoalteromonas sp. 643A was determined in a covalently inhibited form at a resolution of 1.35 A. The enzyme has a typical SGNH hydrolase structure consisting of a single domain containing a five-stranded beta-sheet, with three helices at the convex side and two helices at the concave side of the sheet, and is ornamented with a couple of very short helices at the domain edges. The active site is located in a groove and contains the classic catalytic triad of Ser, His and Asp. In the structure of the crystal soaked in diethyl p-nitrophenyl phosphate (DNP), the catalytic serine is covalently connected to a phosphonate moiety that clearly has only one ethyl group. This is the only example in the Protein Data Bank of a DNP-inhibited enzyme with covalently bound monoethylphosphate.
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{{STRUCTURE_3hp4| PDB=3hp4 | SCENE= }}
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Structure of EstA esterase from psychrotrophic Pseudoalteromonas sp. 643A covalently inhibited by monoethylphosphonate.,Brzuszkiewicz A, Nowak E, Dauter Z, Dauter M, Cieslinski H, Dlugolecka A, Kur J Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009 Sep 1;65(Pt, 9):862-5. Epub 2009 Aug 20. PMID:19724118<ref>PMID:19724118</ref>
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===Crystal structure of psychrotrophic esterase EstA from Pseudoalteromonas sp. 643A inhibited by monoethylphosphonate===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_19724118}}
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== References ==
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<references/>
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==About this Structure==
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__TOC__
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[[3hp4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudoalteromonas_sp. Pseudoalteromonas sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HP4 OCA].
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</StructureSection>
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[[Category: Pseudoalteromonas sp]]
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==Reference==
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[[Category: Brzuszkiewicz, A]]
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<ref group="xtra">PMID:019724118</ref><references group="xtra"/>
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[[Category: Cieslinski, H]]
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[[Category: Pseudoalteromonas sp.]]
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[[Category: Dauter, M]]
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[[Category: Brzuszkiewicz, A.]]
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[[Category: Dauter, Z]]
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[[Category: Cieslinski, H.]]
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[[Category: Kur, J]]
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[[Category: Dauter, M.]]
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[[Category: Nowak, E]]
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[[Category: Dauter, Z.]]
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[[Category: Kur, J.]]
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[[Category: Nowak, E.]]
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[[Category: Esterase]]
[[Category: Esterase]]
[[Category: Hydrolase]]
[[Category: Hydrolase]]
[[Category: Monoethylphosphonate]]
[[Category: Monoethylphosphonate]]
[[Category: Psychrotrophic]]
[[Category: Psychrotrophic]]

Revision as of 08:45, 8 December 2014

Crystal structure of psychrotrophic esterase EstA from Pseudoalteromonas sp. 643A inhibited by monoethylphosphonate

3hp4, resolution 1.35Å

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