3j1o
From Proteopedia
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| - | [[ | + | ==Cryo-EM map of a yeast minimal preinitiation complex interacting with the Mediator Head module== |
| + | <StructureSection load='3j1o' size='340' side='right' caption='[[3j1o]], [[Resolution|resolution]] 16.00Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[3j1o]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Gekko_coi Gekko coi] and [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J1O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3J1O FirstGlance]. <br> | ||
| + | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3j1n|3j1n]]</td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MED11, YMR112C, YM9718.11C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]), SRB4, MED17, YER022W ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]), MED8, YBR193C, YBR1403 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]), SRB6, MED22, YBR253W, YBR1721 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1118509 Gekko coi]), SRB5, MED18, YGR104C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]), SRB2, HRS2, MED20, YHR041C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]), MED6, MTR32, YHR058C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j1o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j1o OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3j1o RCSB], [http://www.ebi.ac.uk/pdbsum/3j1o PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Mediator, a large (21 polypeptides, MW approximately 1 MDa) complex conserved throughout eukaryotes, plays an essential role in control of gene expression by conveying regulatory signals that influence the activity of the preinitiation complex. However, the precise mode of interaction between Mediator and RNA polymerase II (RNAPII), and the mechanism of regulation by Mediator remain elusive. We used cryo-electron microscopy and reconstituted in vitro transcription assays to characterize a transcriptionally-active complex including the Mediator Head module and components of a minimum preinitiation complex (RNAPII, TFIIF, TFIIB, TBP, and promoter DNA). Our results reveal how the Head interacts with RNAPII, affecting its conformation and function. | ||
| - | + | Interaction of the mediator head module with RNA polymerase II.,Cai G, Chaban YL, Imasaki T, Kovacs JA, Calero G, Penczek PA, Takagi Y, Asturias FJ Structure. 2012 May 9;20(5):899-910. doi: 10.1016/j.str.2012.02.023. PMID:22579255<ref>PMID:22579255</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| - | + | <references/> | |
| - | == | + | __TOC__ |
| - | + | </StructureSection> | |
[[Category: Gekko coi]] | [[Category: Gekko coi]] | ||
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
| - | [[Category: Asturias, F J | + | [[Category: Asturias, F J]] |
| - | [[Category: Imasaki, T | + | [[Category: Imasaki, T]] |
[[Category: Mediator head module]] | [[Category: Mediator head module]] | ||
[[Category: Preinitiation complex]] | [[Category: Preinitiation complex]] | ||
[[Category: Rna polymerase ii]] | [[Category: Rna polymerase ii]] | ||
[[Category: Transcription]] | [[Category: Transcription]] | ||
Revision as of 08:49, 8 December 2014
Cryo-EM map of a yeast minimal preinitiation complex interacting with the Mediator Head module
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