2xb0
From Proteopedia
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| - | [[ | + | ==DNA-binding domain from Saccharomyces cerevisiae chromatin- remodelling protein Chd1== |
| + | <StructureSection load='2xb0' size='340' side='right' caption='[[2xb0]], [[Resolution|resolution]] 2.00Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[2xb0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XB0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XB0 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xb0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xb0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2xb0 RCSB], [http://www.ebi.ac.uk/pdbsum/2xb0 PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The ATP-dependent chromatin-remodelling enzyme Chd1 is a 168-kDa protein consisting of a double chromodomain, Snf2-related ATPase domain, and a C-terminal DNA-binding domain. Here, we show the DNA-binding domain is required for Saccharomyces cerevisiae Chd1 to bind and remodel nucleosomes. The crystal structure of this domain reveals the presence of structural homology to SANT and SLIDE domains previously identified in ISWI remodelling enzymes. The presence of these domains in ISWI and Chd1 chromatin-remodelling enzymes may provide a means of efficiently harnessing the action of the Snf2-related ATPase domain for the purpose of nucleosome spacing and provide an explanation for partial redundancy between these proteins. Site directed mutagenesis was used to identify residues important for DNA binding and generate a model describing the interaction of this domain with DNA. Through inclusion of Chd1 sequences in homology searches SLIDE domains were identified in CHD6-9 proteins. Point mutations to conserved amino acids within the human CHD7 SLIDE domain have been identified in patients with CHARGE syndrome. | ||
| - | + | The DNA-binding domain of the Chd1 chromatin-remodelling enzyme contains SANT and SLIDE domains.,Ryan DP, Sundaramoorthy R, Martin D, Singh V, Owen-Hughes T EMBO J. 2011 May 27. PMID:21623345<ref>PMID:21623345</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
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==See Also== | ==See Also== | ||
*[[Helicase|Helicase]] | *[[Helicase|Helicase]] | ||
| - | + | == References == | |
| - | == | + | <references/> |
| - | < | + | __TOC__ |
| + | </StructureSection> | ||
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
| - | [[Category: Owen-Hughes, T | + | [[Category: Owen-Hughes, T]] |
| - | [[Category: Ryan, D P | + | [[Category: Ryan, D P]] |
| - | [[Category: Sundaramoorthy, R | + | [[Category: Sundaramoorthy, R]] |
[[Category: Chromatin regulator]] | [[Category: Chromatin regulator]] | ||
[[Category: Dna-binding protein]] | [[Category: Dna-binding protein]] | ||
[[Category: Hydrolase]] | [[Category: Hydrolase]] | ||
[[Category: Transcription]] | [[Category: Transcription]] | ||
Revision as of 08:52, 8 December 2014
DNA-binding domain from Saccharomyces cerevisiae chromatin- remodelling protein Chd1
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