3ai1
From Proteopedia
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| - | [[ | + | ==The crystal structure of L-sorbose reductase from Gluconobacter frateurii complexed with NADPH and L-sorbose reveals the structure bases of its catalytic mechanism and high substrate selectivity== |
| + | <StructureSection load='3ai1' size='340' side='right' caption='[[3ai1]], [[Resolution|resolution]] 2.38Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[3ai1]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Gluconobacter_frateurii Gluconobacter frateurii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AI1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3AI1 FirstGlance]. <br> | ||
| + | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3ai2|3ai2]], [[3ai3|3ai3]]</td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">sboA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=38308 Gluconobacter frateurii])</td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Sorbose_reductase Sorbose reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.289 1.1.1.289] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ai1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ai1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ai1 RCSB], [http://www.ebi.ac.uk/pdbsum/3ai1 PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | l-Sorbose reductase from Gluconobacter frateurii (SR) is an NADPH-dependent oxidoreductase. SR preferentially catalyzes the reversible reaction between d-sorbitol and l-sorbose with high substrate specificity. To elucidate the structural basis of the catalytic mechanism and the substrate specificity of SR, we have determined the structures of apo-SR, SR in complex with NADPH, and the inactive mutant (His116Leu) of SR in complex with NADPH and l-sorbose at 2.83 A, 1.90 A, and 1.80 A resolutions, respectively. Our results show that SR belongs to the short-chain dehydrogenase/reductase (SDR) family and forms a tetrameric structure. Although His116 is not conserved among SDR family enzymes, the structures of SR have revealed that His116 is important for the stabilization of the proton relay system and for active-site conformation as a fourth catalytic residue. In the ternary complex structure, l-sorbose is recognized by 11 hydrogen bonds. Site-directed mutagenesis of residues around the l-sorbose-binding site has shown that the loss of almost full enzymatic activity was caused by not only the substitution of putative catalytic residues but also the substitution of the residue used for the recognition of the C4 hydroxyl groups of l-sorbose (Glu154) and of the residues used for the construction of the substrate-binding pocket (Cys146 and Gly188). The recognition of the C4 hydroxyl group of l-sorbose would be indispensable for the substrate specificity of SR, which recognizes only l-sorbose and d-sorbitol but not other sugars. Our results indicated that these residues were crucial for the substrate recognition and specificity of SR. | ||
| - | + | The crystal structure of l-sorbose reductase from Gluconobacter frateurii complexed with NADPH and l-sorbose.,Kubota K, Nagata K, Okai M, Miyazono K, Soemphol W, Ohtsuka J, Yamamura A, Saichana N, Toyama H, Matsushita K, Tanokura M J Mol Biol. 2011 Apr 8;407(4):543-55. Epub 2011 Jan 26. PMID:21277857<ref>PMID:21277857</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| - | + | <references/> | |
| - | + | __TOC__ | |
| - | + | </StructureSection> | |
| - | + | ||
| - | == | + | |
| - | < | + | |
[[Category: Gluconobacter frateurii]] | [[Category: Gluconobacter frateurii]] | ||
[[Category: Sorbose reductase]] | [[Category: Sorbose reductase]] | ||
| - | [[Category: Kubota, K | + | [[Category: Kubota, K]] |
| - | [[Category: Miyazono, K | + | [[Category: Miyazono, K]] |
| - | [[Category: Nagata, K | + | [[Category: Nagata, K]] |
| - | [[Category: Okai, M | + | [[Category: Okai, M]] |
| - | [[Category: Tanokura, M | + | [[Category: Tanokura, M]] |
[[Category: Nadph-dependent reductase]] | [[Category: Nadph-dependent reductase]] | ||
[[Category: Oxidoreductase]] | [[Category: Oxidoreductase]] | ||
[[Category: Rossmann-fold]] | [[Category: Rossmann-fold]] | ||
[[Category: Short chain dehydrogenase/reductase]] | [[Category: Short chain dehydrogenase/reductase]] | ||
Revision as of 08:52, 8 December 2014
The crystal structure of L-sorbose reductase from Gluconobacter frateurii complexed with NADPH and L-sorbose reveals the structure bases of its catalytic mechanism and high substrate selectivity
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