3j09
From Proteopedia
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| - | [[ | + | ==High resolution helical reconstruction of the bacterial p-type ATPase copper transporter CopA== |
| + | <StructureSection load='3j09' size='340' side='right' caption='[[3j09]], [[Resolution|resolution]] 10.00Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[3j09]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J09 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3J09 FirstGlance]. <br> | ||
| + | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">copA, pacS, AF_0473 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2234 Archaeoglobus fulgidus])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j09 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j09 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3j09 RCSB], [http://www.ebi.ac.uk/pdbsum/3j09 PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | CopA uses ATP to pump Cu(+) across cell membranes. X-ray crystallography has defined atomic structures of several related P-type ATPases. We have determined a structure of CopA at 10 A resolution by cryo-electron microscopy of a new crystal form and used computational molecular docking to study the interactions between the N-terminal metal-binding domain (NMBD) and other elements of the molecule. We found that the shorter-chain lipids used to produce these crystals are associated with movements of the cytoplasmic domains, with a novel dimer interface and with disordering of the NMBD, thus offering evidence for the transience of its interaction with the other cytoplasmic domains. Docking identified a binding site that matched the location of the NMBD in our previous structure by cryo-electron microscopy, allowing a more detailed view of its binding configuration and further support for its role in autoinhibition. | ||
| - | + | The Architecture of CopA from Archeaoglobus fulgidus Studied by Cryo-Electron Microscopy and Computational Docking.,Allen GS, Wu CC, Cardozo T, Stokes DL Structure. 2011 Aug 3. PMID:21820315<ref>PMID:21820315</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
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==See Also== | ==See Also== | ||
*[[ATPase|ATPase]] | *[[ATPase|ATPase]] | ||
| - | + | == References == | |
| - | == | + | <references/> |
| - | < | + | __TOC__ |
| + | </StructureSection> | ||
[[Category: Archaeoglobus fulgidus]] | [[Category: Archaeoglobus fulgidus]] | ||
| - | [[Category: Allen, G S | + | [[Category: Allen, G S]] |
| - | [[Category: Cardozo, T | + | [[Category: Cardozo, T]] |
| - | [[Category: Stokes, D L | + | [[Category: Stokes, D L]] |
| - | [[Category: Wu, C | + | [[Category: Wu, C]] |
[[Category: Adenosine triphosphatase]] | [[Category: Adenosine triphosphatase]] | ||
[[Category: Archaeal protein]] | [[Category: Archaeal protein]] | ||
Revision as of 08:52, 8 December 2014
High resolution helical reconstruction of the bacterial p-type ATPase copper transporter CopA
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