3gl4

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[[Image:3gl4.png|left|200px]]
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==X-ray structure of photobleached killerred==
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<StructureSection load='3gl4' size='340' side='right' caption='[[3gl4]], [[Resolution|resolution]] 2.15&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3gl4]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Eukaryota Eukaryota]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GL4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3GL4 FirstGlance]. <br>
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</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CRQ:[2-(3-CARBAMOYL-1-IMINO-PROPYL)-4-(4-HYDROXY-BENZYLIDENE)-5-OXO-4,5-DIHYDRO-IMIDAZOL-1-YL]-ACETIC+ACID'>CRQ</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3gb3|3gb3]]</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3gl4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gl4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3gl4 RCSB], [http://www.ebi.ac.uk/pdbsum/3gl4 PDBsum]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gl/3gl4_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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KillerRed is the only known fluorescent protein that demonstrates notable phototoxicity, exceeding that of the other green and red fluorescent proteins by at least 1,000-fold. KillerRed could serve as an instrument to inactivate target proteins or to kill cell populations in photodynamic therapy. However, the nature of KillerRed phototoxicity has remained unclear, impeding the development of more phototoxic variants. Here we present the results of a high resolution crystallographic study of KillerRed in the active fluorescent and in the photobleached non-fluorescent states. A unique and striking feature of the structure is a water-filled channel reaching the chromophore area from the end cap of the beta-barrel that is probably one of the key structural features responsible for phototoxicity. A study of the structure-function relationship of KillerRed, supported by structure-based, site-directed mutagenesis, has also revealed the key residues most likely responsible for the phototoxic effect. In particular, Glu(68) and Ser(119), located adjacent to the chromophore, have been assigned as the primary trigger of the reaction chain.
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{{STRUCTURE_3gl4| PDB=3gl4 | SCENE= }}
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Structural basis for phototoxicity of the genetically encoded photosensitizer KillerRed.,Pletnev S, Gurskaya NG, Pletneva NV, Lukyanov KA, Chudakov DM, Martynov VI, Popov VO, Kovalchuk MV, Wlodawer A, Dauter Z, Pletnev V J Biol Chem. 2009 Nov 13;284(46):32028-39. Epub 2009 Sep 8. PMID:19737938<ref>PMID:19737938</ref>
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===X-ray structure of photobleached killerred===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_19737938}}
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== References ==
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<references/>
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==About this Structure==
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__TOC__
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[[3gl4]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Eukaryota Eukaryota]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GL4 OCA].
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</StructureSection>
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==Reference==
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<ref group="xtra">PMID:019737938</ref><references group="xtra"/>
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[[Category: Eukaryota]]
[[Category: Eukaryota]]
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[[Category: Pletnev, S.]]
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[[Category: Pletnev, S]]
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[[Category: Pletnev, V Z.]]
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[[Category: Pletnev, V Z]]
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[[Category: Pletneva, N V.]]
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[[Category: Pletneva, N V]]
[[Category: Fluorescent protein]]
[[Category: Fluorescent protein]]
[[Category: Genetically encoded photosensitizer]]
[[Category: Genetically encoded photosensitizer]]
[[Category: Killerred]]
[[Category: Killerred]]
[[Category: Phototoxicity]]
[[Category: Phototoxicity]]

Revision as of 08:58, 8 December 2014

X-ray structure of photobleached killerred

3gl4, resolution 2.15Å

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