3h8l
From Proteopedia
(Difference between revisions)
m (Protected "3h8l" [edit=sysop:move=sysop]) |
|||
| Line 1: | Line 1: | ||
| - | [[ | + | ==The first X-ray structure of a sulfide:quinone oxidoreductase: insights into sulfide oxidation mechanism== |
| + | <StructureSection load='3h8l' size='340' side='right' caption='[[3h8l]], [[Resolution|resolution]] 2.57Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[3h8l]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Acidianus_ambivalens Acidianus ambivalens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3H8L OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3H8L FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=S3H:TRISULFANE'>S3H</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3h8i|3h8i]]</td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/NADH_dehydrogenase NADH dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.6.99.3 1.6.99.3] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3h8l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3h8l OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3h8l RCSB], [http://www.ebi.ac.uk/pdbsum/3h8l PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h8/3h8l_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | A sulfide:quinone oxidoreductase (SQR) was isolated from the membranes of the hyperthermoacidophilic archaeon Acidianus ambivalens, and its X-ray structure, the first reported for an SQR, was determined to 2.6 A resolution. This enzyme was functionally and structurally characterized and was shown to have two redox active sites: a covalently bound FAD and an adjacent pair of cysteine residues. Most interestingly, the X-ray structure revealed the presence of a chain of three sulfur atoms bridging those two cysteine residues. The possible implications of this observation in the catalytic mechanism for sulfide oxidation are discussed, and the role of SQR in the sulfur dependent bioenergetics of A. ambivalens, linked to oxygen reduction, is addressed. | ||
| - | + | Structural and functional insights into sulfide:quinone oxidoreductase.,Brito JA, Sousa FL, Stelter M, Bandeiras TM, Vonrhein C, Teixeira M, Pereira MM, Archer M Biochemistry. 2009 Jun 23;48(24):5613-22. PMID:19438211<ref>PMID:19438211</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| - | + | <references/> | |
| - | + | __TOC__ | |
| - | + | </StructureSection> | |
| - | + | ||
| - | == | + | |
| - | < | + | |
[[Category: Acidianus ambivalens]] | [[Category: Acidianus ambivalens]] | ||
[[Category: NADH dehydrogenase]] | [[Category: NADH dehydrogenase]] | ||
| - | [[Category: Archer, M | + | [[Category: Archer, M]] |
| - | [[Category: Bandeiras, T M | + | [[Category: Bandeiras, T M]] |
| - | [[Category: Brito, J A | + | [[Category: Brito, J A]] |
| - | [[Category: Pereira, M M | + | [[Category: Pereira, M M]] |
| - | [[Category: Sousa, F L | + | [[Category: Sousa, F L]] |
| - | [[Category: Stelter, M | + | [[Category: Stelter, M]] |
| - | [[Category: Teixeira, M | + | [[Category: Teixeira, M]] |
| - | [[Category: Vonrhein, C | + | [[Category: Vonrhein, C]] |
[[Category: Complete form]] | [[Category: Complete form]] | ||
[[Category: Membrane protein]] | [[Category: Membrane protein]] | ||
[[Category: Oxidoreductase]] | [[Category: Oxidoreductase]] | ||
[[Category: Rossman-like fold]] | [[Category: Rossman-like fold]] | ||
Revision as of 08:58, 8 December 2014
The first X-ray structure of a sulfide:quinone oxidoreductase: insights into sulfide oxidation mechanism
| |||||||||||
