3gyv

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[[Image:3gyv.png|left|200px]]
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==Crystal structure of nucleosome assembly protein from Plasmodium falciparum==
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<StructureSection load='3gyv' size='340' side='right' caption='[[3gyv]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3gyv]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Plasmodium_falciparum Plasmodium falciparum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GYV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3GYV FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3fs3|3fs3]], [[3gyw|3gyw]]</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PFL0185c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5833 Plasmodium falciparum])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3gyv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gyv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3gyv RCSB], [http://www.ebi.ac.uk/pdbsum/3gyv PDBsum]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gy/3gyv_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The crystal structure of Plasmodium falciparum nucleosome assembly protein (PfNapL) was determined by iodide-SAD/SIRAS phasing methods using iodide-SAD data to 3.0 A resolution and native data to 2.4 A resolution. Halide-derivatized PfNapL crystals were obtained using the quick cryo-soaking method in which the native crystals were soaked in a cryosolution consisting of 500 mM NaI for a short period of 30-60 s and data were collected at an in-house X-ray source using Cu Kalpha radiation. Despite a low anomalous signal-to-noise ratio of &lt;1.2 in the &gt;3.5 A resolution bin, the data were sufficient to determine the structure by SAD/SIR/SIRAS methods using the soaked iodides. Previously, structure solution had failed with both molecular-replacement and selenomethionine-derivatization techniques owing to reasons that are detailed in this work. The phasing at low resolution with three iodides per monomer with high temperature factors was successful using any of the SAD, SIR or SIRAS methods.
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{{STRUCTURE_3gyv| PDB=3gyv | SCENE= }}
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Iodide-SAD, SIR and SIRAS phasing for structure solution of a nucleosome assembly protein.,Yogavel M, Gill J, Sharma A Acta Crystallogr D Biol Crystallogr. 2009 Jun;65(Pt 6):618-22. Epub 2009, May 15. PMID:19465776<ref>PMID:19465776</ref>
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===Crystal structure of nucleosome assembly protein from Plasmodium falciparum===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_19465776}}
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== References ==
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<references/>
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==About this Structure==
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__TOC__
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[[3gyv]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Plasmodium_falciparum Plasmodium falciparum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GYV OCA].
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</StructureSection>
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==Reference==
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<ref group="xtra">PMID:019465776</ref><references group="xtra"/>
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[[Category: Plasmodium falciparum]]
[[Category: Plasmodium falciparum]]
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[[Category: Gill, J.]]
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[[Category: Gill, J]]
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[[Category: Sharma, A.]]
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[[Category: Sharma, A]]
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[[Category: Yogavel, M.]]
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[[Category: Yogavel, M]]
[[Category: Chaperone]]
[[Category: Chaperone]]
[[Category: Histone recognition]]
[[Category: Histone recognition]]
[[Category: Iodide-sad]]
[[Category: Iodide-sad]]
[[Category: Nucleosome assembly protein]]
[[Category: Nucleosome assembly protein]]

Revision as of 09:03, 8 December 2014

Crystal structure of nucleosome assembly protein from Plasmodium falciparum

3gyv, resolution 3.00Å

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