3hjk

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[[Image:3hjk.png|left|200px]]
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==2.0 Angstrom Structure of the Ile74Val Variant of Vivid (VVD).==
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<StructureSection load='3hjk' size='340' side='right' caption='[[3hjk]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3hjk]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Neurospora_crassa Neurospora crassa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HJK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3HJK FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3hji|3hji]], [[2pd7|2pd7]], [[2pd8|2pd8]], [[2pdt|2pdt]], [[2pdr|2pdr]], [[3d72|3d72]]</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">G17A4.050, Vivid, vvd ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5141 Neurospora crassa])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hjk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hjk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3hjk RCSB], [http://www.ebi.ac.uk/pdbsum/3hjk PDBsum]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hj/3hjk_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Phototropin-like LOV domains form a cysteinyl-flavin adduct in response to blue light but show considerable variation in output signal and the lifetime of the photo-adduct signaling state. Mechanistic studies of the slow-cycling fungal LOV photoreceptor Vivid (VVD) reveal the importance of reactive cysteine conformation, flavin electronic environment and solvent accessibility for adduct scission and thermal reversion. Proton inventory, pH effects, base catalysis and structural studies implicate flavin N(5) deprotonation as rate-determining for recovery. Substitutions of active site residues Ile74, Ile85, Met135 and Met165 alter photoadduct lifetimes by over four orders of magnitude in VVD, and similar changes in other LOV proteins show analogous effects. Adduct state decay rates also correlate with changes in conformational and oligomeric properties of the protein necessary for signaling. These findings link natural sequence variation of LOV domains to function and provide a means to design broadly reactive light-sensitive probes.
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{{STRUCTURE_3hjk| PDB=3hjk | SCENE= }}
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Mechanism-based tuning of a LOV domain photoreceptor.,Zoltowski BD, Vaccaro B, Crane BR Nat Chem Biol. 2009 Nov;5(11):827-34. Epub 2009 Aug 30. PMID:19718042<ref>PMID:19718042</ref>
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===2.0 Angstrom Structure of the Ile74Val Variant of Vivid (VVD).===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_19718042}}
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== References ==
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<references/>
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==About this Structure==
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__TOC__
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[[3hjk]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Neurospora_crassa Neurospora crassa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HJK OCA].
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</StructureSection>
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==Reference==
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<ref group="xtra">PMID:019718042</ref><references group="xtra"/>
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[[Category: Neurospora crassa]]
[[Category: Neurospora crassa]]
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[[Category: Crane, B R.]]
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[[Category: Crane, B R]]
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[[Category: Vaccaro, B J.]]
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[[Category: Vaccaro, B J]]
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[[Category: Zoltowski, B D.]]
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[[Category: Zoltowski, B D]]
[[Category: Circadian clock]]
[[Category: Circadian clock]]
[[Category: Fad]]
[[Category: Fad]]

Revision as of 09:04, 8 December 2014

2.0 Angstrom Structure of the Ile74Val Variant of Vivid (VVD).

3hjk, resolution 2.00Å

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