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3ho6

From Proteopedia

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[[Image:3ho6.png|left|200px]]
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==Structure-function analysis of inositol hexakisphosphate-induced autoprocessing in clostridium difficile toxin A==
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<StructureSection load='3ho6' size='340' side='right' caption='[[3ho6]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3ho6]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/[clostridium]_difficile [clostridium] difficile]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HO6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3HO6 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IHP:INOSITOL+HEXAKISPHOSPHATE'>IHP</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">tcdA, toxA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1496 [Clostridium] difficile])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ho6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ho6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ho6 RCSB], [http://www.ebi.ac.uk/pdbsum/3ho6 PDBsum]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ho/3ho6_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The action of Clostridium difficile toxins A and B depends on inactivation of host small G-proteins by glucosylation. Cellular inositol hexakisphosphate (InsP6) induces an autocatalytic cleavage of the toxins, releasing an N-terminal glucosyltransferase domain into the host cell cytosol. We have defined the cysteine protease domain (CPD) responsible for autoprocessing within toxin A (TcdA) and report the 1.6 A x-ray crystal structure of the domain bound to InsP6. InsP6 is bound in a highly basic pocket that is separated from an unusual active site by a beta-flap structure. Functional studies confirm an intramolecular mechanism of cleavage and highlight specific residues required for InsP6-induced TcdA processing. Analysis of the structural and functional data in the context of sequences from similar and diverse origins highlights a C-terminal extension and a pi-cation interaction within the beta-flap that appear to be unique among the large clostridial cytotoxins.
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{{STRUCTURE_3ho6| PDB=3ho6 | SCENE= }}
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Structure-function analysis of inositol hexakisphosphate-induced autoprocessing in Clostridium difficile toxin A.,Pruitt RN, Chagot B, Cover M, Chazin WJ, Spiller B, Lacy DB J Biol Chem. 2009 Aug 14;284(33):21934-40. Epub 2009 Jun 24. PMID:19553670<ref>PMID:19553670</ref>
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===Structure-function analysis of inositol hexakisphosphate-induced autoprocessing in clostridium difficile toxin A===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_19553670}}
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== References ==
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<references/>
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==About this Structure==
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__TOC__
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[[3ho6]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/[clostridium]_difficile [clostridium] difficile]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HO6 OCA].
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</StructureSection>
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[[Category: Lacy, D B]]
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==Reference==
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[[Category: Pruitt, R N]]
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<ref group="xtra">PMID:019553670</ref><references group="xtra"/>
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[[Category: Lacy, D B.]]
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[[Category: Pruitt, R N.]]
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[[Category: Enterotoxin]]
[[Category: Enterotoxin]]
[[Category: Inositol phosphate]]
[[Category: Inositol phosphate]]
[[Category: Toxin]]
[[Category: Toxin]]

Revision as of 09:10, 8 December 2014

Structure-function analysis of inositol hexakisphosphate-induced autoprocessing in clostridium difficile toxin A

3ho6, resolution 1.60Å

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