1mqf

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[[Image:1mqf.gif|left|200px]]<br /><applet load="1mqf" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1mqf.gif|left|200px]]
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caption="1mqf, resolution 2.50&Aring;" />
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'''Compound I from Proteus mirabilis catalase'''<br />
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{{Structure
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|PDB= 1mqf |SIZE=350|CAPTION= <scene name='initialview01'>1mqf</scene>, resolution 2.50&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=O:OXYGEN+ATOM'>O</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene> and <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Catalase Catalase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.6 1.11.1.6]
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|GENE=
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}}
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'''Compound I from Proteus mirabilis catalase'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1MQF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Proteus_mirabilis Proteus mirabilis] with <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=O:'>O</scene>, <scene name='pdbligand=HEM:'>HEM</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. This structure supersedes the now removed PDB entry 2CAF. Active as [http://en.wikipedia.org/wiki/Catalase Catalase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.6 1.11.1.6] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MQF OCA].
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1MQF is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Proteus_mirabilis Proteus mirabilis]. This structure supersedes the now removed PDB entry 2CAF. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MQF OCA].
==Reference==
==Reference==
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Structural studies of Proteus mirabilis catalase in its ground state, oxidized state and in complex with formic acid., Andreoletti P, Pernoud A, Sainz G, Gouet P, Jouve HM, Acta Crystallogr D Biol Crystallogr. 2003 Dec;59(Pt 12):2163-8. Epub 2003, Nov 27. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14646074 14646074]
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Structural studies of Proteus mirabilis catalase in its ground state, oxidized state and in complex with formic acid., Andreoletti P, Pernoud A, Sainz G, Gouet P, Jouve HM, Acta Crystallogr D Biol Crystallogr. 2003 Dec;59(Pt 12):2163-8. Epub 2003, Nov 27. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14646074 14646074]
[[Category: Catalase]]
[[Category: Catalase]]
[[Category: Proteus mirabilis]]
[[Category: Proteus mirabilis]]
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[[Category: alpha + beta]]
[[Category: alpha + beta]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:57:59 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:46:49 2008''

Revision as of 10:46, 20 March 2008

Image:1mqf.gif


PDB ID 1mqf

Drag the structure with the mouse to rotate
, resolution 2.50Å
Ligands: , , and
Activity: Catalase, with EC number 1.11.1.6
Coordinates: save as pdb, mmCIF, xml



Compound I from Proteus mirabilis catalase


Overview

The structure of Proteus mirabilis catalase in complex with an inhibitor, formic acid, has been solved at 2.3 A resolution. Formic acid is a key ligand of catalase because of its ability to react with the ferric enzyme, giving a high-spin iron complex. Alternatively, it can react with two transient oxidized intermediates of the enzymatic mechanism, compounds I and II. In this work, the structures of native P. mirabilis catalase (PMC) and compound I have also been determined at high resolution (2.0 and 2.5 A, respectively) from frozen crystals. Comparisons between these three PMC structures show that a water molecule present at a distance of 3.5 A from the haem iron in the resting state is absent in the formic acid complex, but reappears in compound I. In addition, movements of solvent molecules are observed during formation of compound I in a cavity located away from the active site, in which a glycerol molecule is replaced by a sulfate. These results give structural insights into the movement of solvent molecules, which may be important in the enzymatic reaction.

About this Structure

1MQF is a Single protein structure of sequence from Proteus mirabilis. This structure supersedes the now removed PDB entry 2CAF. Full crystallographic information is available from OCA.

Reference

Structural studies of Proteus mirabilis catalase in its ground state, oxidized state and in complex with formic acid., Andreoletti P, Pernoud A, Sainz G, Gouet P, Jouve HM, Acta Crystallogr D Biol Crystallogr. 2003 Dec;59(Pt 12):2163-8. Epub 2003, Nov 27. PMID:14646074

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