3jv8
From Proteopedia
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| - | [[ | + | ==Crystal Structure of Lumazine Synthase from Bacillus Anthracis== |
| + | <StructureSection load='3jv8' size='340' side='right' caption='[[3jv8]], [[Resolution|resolution]] 3.50Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[3jv8]] is a 30 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JV8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3JV8 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1vsw|1vsw]], [[1vsx|1vsx]]</td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ribH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1392 Bacillus anthracis])</td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/6,7-dimethyl-8-ribityllumazine_synthase 6,7-dimethyl-8-ribityllumazine synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.78 2.5.1.78] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3jv8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3jv8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3jv8 RCSB], [http://www.ebi.ac.uk/pdbsum/3jv8 PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The crystal structure of lumazine synthase from Bacillus anthracis was solved by molecular replacement and refined to R(cryst) = 23.7% (R(free) = 28.4%) at a resolution of 3.5 A. The structure reveals the icosahedral symmetry of the enzyme and specific features of the active site that are unique in comparison with previously determined orthologues. The application of isothermal titration calorimetry in combination with enzyme kinetics showed that three designed pyrimidine derivatives bind to lumazine synthase with micromolar dissociation constants and competitively inhibit the catalytic reaction. Structure-based modelling suggested the binding modes of the inhibitors in the active site and allowed an estimation of the possible contacts formed upon binding. The results provide a structural framework for the design of antibiotics active against B. anthracis. | ||
| - | + | Structural study and thermodynamic characterization of inhibitor binding to lumazine synthase from Bacillus anthracis.,Morgunova E, Illarionov B, Saller S, Popov A, Sambaiah T, Bacher A, Cushman M, Fischer M, Ladenstein R Acta Crystallogr D Biol Crystallogr. 2010 Sep;66(Pt 9):1001-11. Epub 2010, Aug 13. PMID:20823551<ref>PMID:20823551</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| - | + | <references/> | |
| - | + | __TOC__ | |
| - | + | </StructureSection> | |
| - | + | ||
| - | == | + | |
| - | < | + | |
[[Category: 6,7-dimethyl-8-ribityllumazine synthase]] | [[Category: 6,7-dimethyl-8-ribityllumazine synthase]] | ||
[[Category: Bacillus anthracis]] | [[Category: Bacillus anthracis]] | ||
| - | [[Category: Bacher, A | + | [[Category: Bacher, A]] |
| - | [[Category: Cushman, M | + | [[Category: Cushman, M]] |
| - | [[Category: Fischer, M | + | [[Category: Fischer, M]] |
| - | [[Category: Illarionov, B | + | [[Category: Illarionov, B]] |
| - | [[Category: Ladenstein, R | + | [[Category: Ladenstein, R]] |
| - | [[Category: Morgunova, E | + | [[Category: Morgunova, E]] |
| - | [[Category: Popov, A | + | [[Category: Popov, A]] |
| - | [[Category: Saller, S | + | [[Category: Saller, S]] |
| - | [[Category: Sambaiah, T | + | [[Category: Sambaiah, T]] |
[[Category: Riboflavin biosynthesis]] | [[Category: Riboflavin biosynthesis]] | ||
[[Category: Transferase]] | [[Category: Transferase]] | ||
Revision as of 09:16, 8 December 2014
Crystal Structure of Lumazine Synthase from Bacillus Anthracis
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