8paz
From Proteopedia
| Line 20: | Line 20: | ||
[[Category: electron transfer]] | [[Category: electron transfer]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 17:50:15 2007'' |
Revision as of 15:45, 30 October 2007
|
OXIDIZED NATIVE PSEUDOAZURIN FROM A. FAECALIS
Overview
In order to understand the origins of differences in redox potentials, among cupredoxins (small blue type I copper-containing proteins that, reversibly change oxidation state and interact with redox partners), we, have determined the structures of the native and two mutants (P80A and, P80I) of pseudoazurin from Alcaligenes faecalis S-6 in oxidized and, reduced forms at resolutions of 2.2 A in the worst case and 1.6 A in the, best case. The P80A mutation creates a surface pocket filled by a new, water molecule, whereas the P80I mutant excludes this water. Distinct, patterns of change occur in response to reduction for all three molecules:, the copper position shifts, Met 7 and Pro 35 move, and the relative, orientations of residues 81 to 16, 18 to the amide planes of 77 and 86, all change. ... [(full description)]
About this Structure
8PAZ is a [Single protein] structure of sequence from [Alcaligenes faecalis] with CU as [ligand]. This structure superseeds the now removed PDB entry 2PAZ. Structure known Active Site: CU. Full crystallographic information is available from [OCA].
Reference
Site-directed mutants of pseudoazurin: explanation of increased redox potentials from X-ray structures and from calculation of redox potential differences., Libeu CA, Kukimoto M, Nishiyama M, Horinouchi S, Adman ET, Biochemistry. 1997 Oct 28;36(43):13160-79. PMID:9341204
Page seeded by OCA on Tue Oct 30 17:50:15 2007
