2rra
From Proteopedia
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| - | [[ | + | ==Solution structure of RNA binding domain in human Tra2 beta protein in complex with RNA (GAAGAA)== |
| + | <StructureSection load='2rra' size='340' side='right' caption='[[2rra]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[2rra]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RRA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2RRA FirstGlance]. <br> | ||
| + | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2rrb|2rrb]]</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2rra FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rra OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2rra RCSB], [http://www.ebi.ac.uk/pdbsum/2rra PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Human Transformer2-beta (hTra2-beta) is an important member of the serine/arginine-rich protein family, and contains one RNA recognition motif (RRM). It controls the alternative splicing of several pre-mRNAs, including those of the calcitonin/calcitonin gene-related peptide (CGRP), the survival motor neuron 1 (SMN1) protein and the tau protein. Accordingly, the RRM of hTra2-beta specifically binds to two types of RNA sequences [the CAA and (GAA)(2) sequences]. We determined the solution structure of the hTra2-beta RRM (spanning residues Asn110-Thr201), which not only has a canonical RRM fold, but also an unusual alignment of the aromatic amino acids on the beta-sheet surface. We then solved the complex structure of the hTra2-beta RRM with the (GAA)(2) sequence, and found that the AGAA tetra-nucleotide was specifically recognized through hydrogen-bond formation with several amino acids on the N- and C-terminal extensions, as well as stacking interactions mediated by the unusually aligned aromatic rings on the beta-sheet surface. Further NMR experiments revealed that the hTra2-beta RRM recognizes the CAA sequence when it is integrated in the stem-loop structure. This study indicates that the hTra2-beta RRM recognizes two types of RNA sequences in different RNA binding modes. | ||
| - | + | Structural basis for the dual RNA-recognition modes of human Tra2-beta RRM.,Tsuda K, Someya T, Kuwasako K, Takahashi M, He F, Unzai S, Inoue M, Harada T, Watanabe S, Terada T, Kobayashi N, Shirouzu M, Kigawa T, Tanaka A, Sugano S, Guntert P, Yokoyama S, Muto Y Nucleic Acids Res. 2011 Mar;39(4):1538-53. Epub 2010 Oct 5. PMID:20926394<ref>PMID:20926394</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| - | + | <references/> | |
| - | + | __TOC__ | |
| - | + | </StructureSection> | |
| - | + | ||
| - | == | + | |
| - | < | + | |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
| - | [[Category: Inoue, M | + | [[Category: Inoue, M]] |
| - | [[Category: Kigawa, T | + | [[Category: Kigawa, T]] |
| - | [[Category: Kuwasako, K | + | [[Category: Kuwasako, K]] |
| - | [[Category: Muto, Y | + | [[Category: Muto, Y]] |
| - | [[Category: Shirouzu, M | + | [[Category: Shirouzu, M]] |
| - | [[Category: Someya, T | + | [[Category: Someya, T]] |
| - | [[Category: Sugano, S | + | [[Category: Sugano, S]] |
| - | [[Category: Takahashi, M | + | [[Category: Takahashi, M]] |
| - | [[Category: Terada, T | + | [[Category: Terada, T]] |
| - | [[Category: Tsuda, K | + | [[Category: Tsuda, K]] |
| - | [[Category: Yokoyama, S | + | [[Category: Yokoyama, S]] |
[[Category: National project on protein structural and functional analyse]] | [[Category: National project on protein structural and functional analyse]] | ||
[[Category: Nppsfa]] | [[Category: Nppsfa]] | ||
[[Category: Protein-rna complex]] | [[Category: Protein-rna complex]] | ||
[[Category: Rbd]] | [[Category: Rbd]] | ||
| - | [[Category: | + | [[Category: Structural genomic]] |
[[Category: Rna binding protein-rna complex]] | [[Category: Rna binding protein-rna complex]] | ||
[[Category: Rrm domain]] | [[Category: Rrm domain]] | ||
[[Category: Rsgi]] | [[Category: Rsgi]] | ||
| - | [[Category: Structural genomic]] | ||
Revision as of 09:38, 8 December 2014
Solution structure of RNA binding domain in human Tra2 beta protein in complex with RNA (GAAGAA)
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Categories: Homo sapiens | Inoue, M | Kigawa, T | Kuwasako, K | Muto, Y | Shirouzu, M | Someya, T | Sugano, S | Takahashi, M | Terada, T | Tsuda, K | Yokoyama, S | National project on protein structural and functional analyse | Nppsfa | Protein-rna complex | Rbd | Structural genomic | Rna binding protein-rna complex | Rrm domain | Rsgi
