3ju9
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | [[ | + | ==Crystal structure of a lectin from Canavalia brasiliensis seed (ConBr) complexed with alpha-aminobutyric acid== |
| + | <StructureSection load='3ju9' size='340' side='right' caption='[[3ju9]], [[Resolution|resolution]] 2.10Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[3ju9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Canavalia_brasiliensis Canavalia brasiliensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JU9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3JU9 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=DBB:D-ALPHA-AMINOBUTYRIC+ACID'>DBB</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1azd|1azd]]</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ju9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ju9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ju9 RCSB], [http://www.ebi.ac.uk/pdbsum/3ju9 PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Diocleinae lectins are highly homologous in their primary structure which features metal binding sites and a carbohydrate recognition domain (CRD). Differences in the biological activity of legume lectins have been widely investigated using hemagglutination inhibition assays, isothermal titration microcalorimetry and co-crystallization with mono- and oligosaccharides. Here we report a new lectin crystal structure (ConBr) extracted from seeds of Canavalia brasiliensis, predict dimannoside binding by docking, identify the alpha-aminobutyric acid (Abu) binding pocket and compare the CRD of ConBr to that of homologous lectins. Based on the hypothesis that the carbohydrate affinity of lectins depends on CRD configuration, the relationship between tridimensional structure and endothelial NO synthase activation was used to clarify differences in biological activity. Our study established a correlation between the position of CRD amino acid side chains and the stimulation of NO release from endothelium. | ||
| - | + | Structural analysis of ConBr reveals molecular correlation between the carbohydrate recognition domain and endothelial NO synthase activation.,Bezerra EH, Rocha BA, Nagano CS, Bezerra Gde A, Moura TR, Bezerra MJ, Benevides RG, Sampaio AH, Assreuy AM, Delatorre P, Cavada BS Biochem Biophys Res Commun. 2011 May 20;408(4):566-70. Epub 2011 Apr 19. PMID:21530490<ref>PMID:21530490</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
==See Also== | ==See Also== | ||
*[[Concanavalin A|Concanavalin A]] | *[[Concanavalin A|Concanavalin A]] | ||
| - | + | == References == | |
| - | == | + | <references/> |
| - | < | + | __TOC__ |
| + | </StructureSection> | ||
[[Category: Canavalia brasiliensis]] | [[Category: Canavalia brasiliensis]] | ||
| - | [[Category: Benevides, R G | + | [[Category: Benevides, R G]] |
| - | [[Category: Bezerra, E H.S | + | [[Category: Bezerra, E H.S]] |
| - | [[Category: Bezerra, G A | + | [[Category: Bezerra, G A]] |
| - | [[Category: Bezerra, M J.B | + | [[Category: Bezerra, M J.B]] |
| - | [[Category: Cavada, B S | + | [[Category: Cavada, B S]] |
| - | [[Category: Delatorre, P | + | [[Category: Delatorre, P]] |
| - | [[Category: Marinho, E S | + | [[Category: Marinho, E S]] |
| - | [[Category: Moura, T R | + | [[Category: Moura, T R]] |
| - | [[Category: Nagano, C S | + | [[Category: Nagano, C S]] |
| - | [[Category: Rocha, B A.M | + | [[Category: Rocha, B A.M]] |
[[Category: Agglutinin]] | [[Category: Agglutinin]] | ||
| - | [[Category: Canavalia brasiliensis]] | ||
[[Category: Conbr]] | [[Category: Conbr]] | ||
[[Category: Lectin]] | [[Category: Lectin]] | ||
Revision as of 09:40, 8 December 2014
Crystal structure of a lectin from Canavalia brasiliensis seed (ConBr) complexed with alpha-aminobutyric acid
| |||||||||||
