1mw9
From Proteopedia
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| - | [[Image:1mw9.gif|left|200px]] | + | [[Image:1mw9.gif|left|200px]] |
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| - | '''Crystal Structure of H365R mutant of 67 kDA N-terminal fragment of E. coli DNA Topoisomerase I''' | + | {{Structure |
| + | |PDB= 1mw9 |SIZE=350|CAPTION= <scene name='initialview01'>1mw9</scene>, resolution 1.67Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/DNA_topoisomerase DNA topoisomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.99.1.2 5.99.1.2] | ||
| + | |GENE= TopA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal Structure of H365R mutant of 67 kDA N-terminal fragment of E. coli DNA Topoisomerase I''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1MW9 is a [ | + | 1MW9 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MW9 OCA]. |
==Reference== | ==Reference== | ||
| - | Structure of a complex between E. coli DNA topoisomerase I and single-stranded DNA., Perry K, Mondragon A, Structure. 2003 Nov;11(11):1349-58. PMID:[http:// | + | Structure of a complex between E. coli DNA topoisomerase I and single-stranded DNA., Perry K, Mondragon A, Structure. 2003 Nov;11(11):1349-58. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14604525 14604525] |
[[Category: DNA topoisomerase]] | [[Category: DNA topoisomerase]] | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
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[[Category: toprim domain]] | [[Category: toprim domain]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:49:02 2008'' |
Revision as of 10:49, 20 March 2008
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| , resolution 1.67Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | TopA (Escherichia coli) | ||||||
| Activity: | DNA topoisomerase, with EC number 5.99.1.2 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of H365R mutant of 67 kDA N-terminal fragment of E. coli DNA Topoisomerase I
Overview
In order to gain insights into the mechaism of ssDNA binding and recognition by Escherichia coli DNA topoisomerase I, the structure of the 67 kDa N-terminal fragment of topoisomerase I was solved in complex with ssDNA. The structure reveals a new conformational stage in the multistep catalytic cycle of type IA topoisomerases. In the structure, the ssDNA binding groove leading to the active site is occupied, but the active site is not fully formed. Large conformational changes are not seen; instead, a single helix parallel to the ssDNA binding groove shifts to clamp the ssDNA. The structure helps clarify the temporal sequence of conformational events, starting from an initial empty enzyme and proceeding to a ssDNA-occupied and catalytically competent active site.
About this Structure
1MW9 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Structure of a complex between E. coli DNA topoisomerase I and single-stranded DNA., Perry K, Mondragon A, Structure. 2003 Nov;11(11):1349-58. PMID:14604525
Page seeded by OCA on Thu Mar 20 12:49:02 2008
