1mx0
From Proteopedia
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| - | [[Image:1mx0.gif|left|200px]] | + | [[Image:1mx0.gif|left|200px]] |
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| - | '''Structure of topoisomerase subunit''' | + | {{Structure |
| + | |PDB= 1mx0 |SIZE=350|CAPTION= <scene name='initialview01'>1mx0</scene>, resolution 2.300Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene> and <scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER'>ANP</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/DNA_topoisomerase_(ATP-hydrolyzing) DNA topoisomerase (ATP-hydrolyzing)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.99.1.3 5.99.1.3] | ||
| + | |GENE= top6b ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2286 Sulfolobus shibatae]) | ||
| + | }} | ||
| + | |||
| + | '''Structure of topoisomerase subunit''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1MX0 is a [ | + | 1MX0 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Sulfolobus_shibatae Sulfolobus shibatae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MX0 OCA]. |
==Reference== | ==Reference== | ||
| - | Structure of the topoisomerase VI-B subunit: implications for type II topoisomerase mechanism and evolution., Corbett KD, Berger JM, EMBO J. 2003 Jan 2;22(1):151-63. PMID:[http:// | + | Structure of the topoisomerase VI-B subunit: implications for type II topoisomerase mechanism and evolution., Corbett KD, Berger JM, EMBO J. 2003 Jan 2;22(1):151-63. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12505993 12505993] |
[[Category: DNA topoisomerase (ATP-hydrolyzing)]] | [[Category: DNA topoisomerase (ATP-hydrolyzing)]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: topoisomerase]] | [[Category: topoisomerase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:49:15 2008'' |
Revision as of 10:49, 20 March 2008
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| , resolution 2.300Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , and | ||||||
| Gene: | top6b (Sulfolobus shibatae) | ||||||
| Activity: | DNA topoisomerase (ATP-hydrolyzing), with EC number 5.99.1.3 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structure of topoisomerase subunit
Overview
Type IIA and type IIB topoisomerases each possess the ability to pass one DNA duplex through another in an ATP-dependent manner. The role of ATP in the strand passage reaction is poorly understood, particularly for the type IIB (topoisomerase VI) family. We have solved the structure of the ATP-binding subunit of topoisomerase VI (topoVI-B) in two states: an unliganded monomer and a nucleotide-bound dimer. We find that topoVI-B is highly structurally homologous to the entire 40-43 kDa ATPase region of type IIA topoisomerases and MutL proteins. Nucleotide binding to topoVI-B leads to dimerization of the protein and causes dramatic conformational changes within each protomer. Our data demonstrate that type IIA and type IIB topoisomerases have descended from a common ancestor and reveal how ATP turnover generates structural signals in the reactions of both type II topoisomerase families. When combined with the structure of the A subunit to create a picture of the intact topoisomerase VI holoenzyme, the ATP-driven motions of topoVI-B reveal a simple mechanism for strand passage by the type IIB topoisomerases.
About this Structure
1MX0 is a Single protein structure of sequence from Sulfolobus shibatae. Full crystallographic information is available from OCA.
Reference
Structure of the topoisomerase VI-B subunit: implications for type II topoisomerase mechanism and evolution., Corbett KD, Berger JM, EMBO J. 2003 Jan 2;22(1):151-63. PMID:12505993
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